Yuan Xiaolong, Li Yunqing, Wang Yi
Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming, People's Republic of China.
Mitochondrial DNA B Resour. 2019 Dec 18;5(1):331-332. doi: 10.1080/23802359.2019.1703604.
The first complete chloroplast genome (cpDNA) sequence of was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 154,010 bp in length, contains a large single copy region (LSC) of 93,676 bp and a small single copy region (SSC) of 18,830 bp, which were separated by a pair of inverted repeats (IR) regions of 20,752 bp. The genome contains 127 genes, including 82 protein-coding genes, 8 ribosomal RNA genes, and 36 transfer RNA genes. The overall GC content of the whole genome is 39.2%, and the corresponding values of the LSC, SSC, and IR regions are 37.9%, 33.9%, and 44.3%, respectively. Further phylogenomic analysis showed that and clustered in a clade in Cinnamomum genus.
本研究通过Illumina HiSeq双端测序数据确定了首个完整的叶绿体基因组（cpDNA）序列。该cpDNA长度为154,010 bp，包含一个93,676 bp的大单拷贝区域（LSC）和一个18,830 bp的小单拷贝区域（SSC），它们被一对20,752 bp的反向重复（IR）区域隔开。该基因组包含127个基因，包括82个蛋白质编码基因、8个核糖体RNA基因和36个转移RNA基因。全基因组的总体GC含量为39.2%，LSC、SSC和IR区域的相应值分别为37.9%、33.9%和44.3%。进一步的系统发育基因组分析表明，[物种名称1]和[物种名称2]聚集在樟属的一个分支中。
