Zheng Yuan, Luo Yana, Li Yunqing, Wang Yi
Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming, People's Republic of China.
College of Forestry, Southwest Forestry University, Kunming, Yunnan, People's Republic of China.
Mitochondrial DNA B Resour. 2019 Dec 12;5(1):204-205. doi: 10.1080/23802359.2019.1698997.
The first complete chloroplast genome (cpDNA) sequence of was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 149,016 bp in length, contains a large single copy region (LSC) of 91,915 bp and a small single copy region (SSC) of 17,703 bp, which were separated by a pair of inverted repeats (IR) regions of 19,699 bp. The genome contains 126 genes, including 81 protein-coding genes, eight ribosomal RNA genes, and 36 transfer RNA genes. The overall GC content of the whole genome is 39.0%, and the corresponding values of the LSC, SSC, and IR regions are 37.7%, 34.4%, and 44.4%, respectively. Further phylogenomic analysis showed that clustered in a clade in Lauraceae family.
在本研究中，通过Illumina HiSeq双端测序数据确定了首个完整的叶绿体基因组（cpDNA）序列。该cpDNA长度为149,016 bp，包含一个91,915 bp的大单拷贝区域（LSC）和一个17,703 bp的小单拷贝区域（SSC），它们被一对19,699 bp的反向重复（IR）区域隔开。该基因组包含126个基因，包括81个蛋白质编码基因、8个核糖体RNA基因和36个转运RNA基因。全基因组的总体GC含量为39.0%，LSC、SSC和IR区域的相应值分别为37.7%、34.4%和44.4%。进一步的系统发育基因组分析表明，其聚集在樟科的一个分支中。
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