Kang Hongmei, Wang Yi
Laboratory of Forest Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming, People's Republic of China.
Mitochondrial DNA B Resour. 2019 Dec 5;4(2):3963-3964. doi: 10.1080/23802359.2019.1688718.
The first complete chloroplast genome (cpDNA) sequence of was determined from Illumina HiSeq pair-end sequencing data in this study. The cpDNA is 162,450 bp in length, contains a large single-copy region (LSC) of 90,871 bp and a small single-copy region (SSC) of 21,137 bp, which were separated by a pair of inverted repeats (IR) regions of 25,221 bp. The genome contains 130 genes, including 85 protein-coding genes, 8 ribosomal RNA genes, and 37 transfer RNA genes. The overall GC content of the whole genome is 38.0%, and the corresponding values of the LSC, SSC, and IR regions are 36.2, 32.1, and 43.5%, respectively. Further, phylogenomic analysis showed that and clustered in a clade in family Menispermaceae.
在本研究中,通过Illumina HiSeq双末端测序数据确定了首个完整的叶绿体基因组(cpDNA)序列。该cpDNA长度为162,450 bp,包含一个90,871 bp的大单拷贝区域(LSC)和一个21,137 bp的小单拷贝区域(SSC),它们被一对25,221 bp的反向重复(IR)区域隔开。该基因组包含130个基因,包括85个蛋白质编码基因、8个核糖体RNA基因和37个转运RNA基因。全基因组的总体GC含量为38.0%,LSC、SSC和IR区域的相应值分别为36.2%、32.1%和43.5%。此外,系统发育基因组分析表明,[物种名称1]和[物种名称2]聚集在防己科的一个分支中。