IGEPP, INRAE, Institut Agro, Univ Rennes, Ploudaniel 29260, France.
Germicopa Breeding, Kerguivarch, Chateauneuf Du Faou 29520, France.
Plant Commun. 2020 Jul 25;1(5):100102. doi: 10.1016/j.xplc.2020.100102. eCollection 2020 Sep 14.
Since its discovery as a bacterial adaptive immune system and its development for genome editing in eukaryotes, the CRISPR technology has revolutionized plant research and precision crop breeding. The CRISPR toolbox holds great promise in the production of crops with genetic disease resistance to increase agriculture resilience and reduce chemical crop protection with a strong impact on the environment and public health. In this review, we provide an extensive overview on recent breakthroughs in CRISPR technology, including the newly developed prime editing system that allows precision gene editing in plants. We present how each CRISPR tool can be selected for optimal use in accordance with its specific strengths and limitations, and illustrate how the CRISPR toolbox can foster the development of genetically pathogen-resistant crops for sustainable agriculture.
自发现细菌适应性免疫系统并将其开发用于真核生物的基因组编辑以来，CRISPR 技术彻底改变了植物研究和精准作物育种。CRISPR 工具在生产具有遗传疾病抗性的作物方面具有巨大的潜力，可以提高农业的弹性，减少对环境和公共健康有强烈影响的化学作物保护。在这篇综述中，我们提供了对 CRISPR 技术最新突破的广泛概述，包括新开发的可在植物中进行精确基因编辑的 prime 编辑系统。我们介绍了如何根据每种 CRISPR 工具的特定优势和局限性来选择最佳用途，并说明了 CRISPR 工具如何促进具有遗传抗性的病原体的作物的发展，以实现可持续农业。
