Bourguignon J P, Franchimont P
Acta Endocrinol (Copenh). 1977 Sep;86(1):15-24. doi: 10.1530/acta.0.0860015.
Highly specific and sensitive radioimmunological methods were applied to determine the levels of LH-RH like immunoreactivity in urine previously extracted by spherosil and methanol, and to assay the gonadotrophins, after extraction with acetone. The endogenous urinary LH-RH like immunoreactivity material was identified by chromatography on Sephadex G25, as having physicochemical properties similar to those of the hormone found in unextracted urine after iv injection of synthetic LH-RH, but different from those of the synthetic decapeptide. The LH-RH like immunoreactivity and the gonadotrophins were assayed in daily collected urine during the reproductive cycle of 3 normal women. A midcycle peak of both FSH and LH was found in each subject. No increases of LH-RH like immunoreactivity were found before or concomittant with the gonadotrophins surge. But peaks of urinary LH-RH like immunoreactivity were observed during the luteal phase, without subsequent increase of gonadotrophin secretion.
应用高度特异和灵敏的放射免疫方法，测定先前用球硅和甲醇提取的尿液中促黄体生成激素释放激素（LH-RH）样免疫反应性水平，并在用丙酮提取后测定促性腺激素。通过在葡聚糖凝胶G25上进行色谱分析，鉴定出内源性尿LH-RH样免疫反应性物质，其理化性质与静脉注射合成LH-RH后未提取尿液中发现的激素相似，但与合成十肽不同。在3名正常女性的生殖周期中，对每日收集的尿液进行LH-RH样免疫反应性和促性腺激素检测。在每个受试者中均发现促卵泡生成素（FSH）和促黄体生成素（LH）的周期中期峰值。在促性腺激素激增之前或同时未发现LH-RH样免疫反应性增加。但在黄体期观察到尿LH-RH样免疫反应性峰值，随后促性腺激素分泌未增加。
