Institute of Structural and Molecular Biology, Birkbeck, University College London, London, United Kingdom.
Oxford Particle Imaging Centre, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, United Kingdom.
Acta Crystallogr D Struct Biol. 2021 Jan 1;77(Pt 1):41-47. doi: 10.1107/S2059798320014928.
Structural determination of molecular complexes by cryo-EM requires large, often complex processing of the image data that are initially obtained. Here, TEMPy2, an update of the TEMPy package to process, optimize and assess cryo-EM maps and the structures fitted to them, is described. New optimization routines, comprehensive automated checks and workflows to perform these tasks are described.
通过冷冻电镜（cryo-EM）结构测定需要对初始获得的大量、通常复杂的图像数据进行大量处理。本文描述了 TEMPy2，这是对 TEMPy 软件包的更新，用于处理、优化和评估冷冻电镜映射图以及与之匹配的结构。本文还介绍了新的优化例程、全面的自动检查和执行这些任务的工作流程。
