Chen Zhongli, Wei Liang, Duru Firat, Chen Liang
1Department of Cardiology, Ruijin Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai, China; 2State Key Laboratory of Cardiovascular Disease, Department of Cardiac Surgery, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; 3University Heart Center Zurich, University Heart Center, Zurich, Switzerland; 4Center for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.
Curr Genomics. 2020 Dec;21(8):585-601. doi: 10.2174/1389202921999200604123914.
The cardiac system is a combination of a complex structure, various cells, and versatile specified functions and sophisticated regulatory mechanisms. Moreover, cardiac diseases that encompass a wide range of endogenous conditions, remain a serious health burden worldwide. Recent genome-wide profiling techniques have taken the lead in uncovering a new realm of cell types and molecular programs driving physiological and pathological processes in various organs and diseases. In particular, the emerging technique single-cell RNA sequencing dominates a breakthrough in decoding the cell heterogeneity, phenotype transition, and developmental dynamics in cardiovascular science.
Herein, we review recent advances in single cellular studies of cardiovascular system and summarize new insights provided by single-cell RNA sequencing in heart developmental sciences, stem-cell researches as well as normal or disease-related working mechanisms.
心脏系统是一个由复杂结构、多种细胞、多样特定功能和精密调节机制组成的组合体。此外，涵盖多种内源性病症的心脏疾病在全球范围内仍是一项严重的健康负担。近期的全基因组分析技术率先揭示了驱动各种器官生理和病理过程以及疾病发生的新型细胞类型和分子程序领域。特别是，新兴技术单细胞RNA测序在解码心血管科学中的细胞异质性、表型转变和发育动力学方面取得了突破。
在此，我们综述心血管系统单细胞研究的最新进展，并总结单细胞RNA测序在心脏发育科学、干细胞研究以及正常或疾病相关工作机制方面提供的新见解。
