Chen Shengqin, Sathuvan Malairaj, Zhang Xiao, Zhang Wancong, Tang Shijie, Liu Yang, Cheong Kit-Leong
Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University, Shantou, 515063, Guangdong, People's Republic of China.
Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong, China.
BMC Chem. 2021 Jan 11;15(1):1. doi: 10.1186/s13065-020-00727-w.
Brown seaweed polysaccharides (BSPs) are one of the primary active components from brown seaweed that has a range of pharmaceutical and biomedical applications. However, the quality control of BSPs is a challenge due to their complicated structure and macromolecule. In this study, saccharide mapping based on high-performance liquid chromatography (HPLC), multi-angle laser light scattering, viscometer, and refractive index detector (HPSEC-MALLS-Vis-RID), and Fourier transform infrared (FT-IR) were used to discriminate the polysaccharides from nine different species of brown algae (BA1-9). The results showed that BSPs were composed of β-D-glucans and β-1,3-1,4-glucan linkages. The molecular weight, radius of gyration, and intrinsic viscosity of BSPs were ranging from 1.718 × 10 Da to 6.630 × 10 Da, 30.2 nm to 51.5 nm, and 360.99 mL/g to 865.52 mL/g, respectively. Moreover, α values of BSPs were in the range of 0.635 to 0.971, which indicated a rigid rod chain conformation. The antioxidant activities of BSPs exhibited substantial radical scavenging activities against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radicals, which indicated that the use of BSPs might be a potential approach for antioxidant supplements. Thus, this study gives insights about the structure-function relationship of BSPs, which will be beneficial to improve the quality of polysaccharides derived from marine algae.
褐藻多糖（BSPs）是褐藻的主要活性成分之一，具有一系列制药和生物医学应用。然而，由于其结构复杂且为大分子，BSPs的质量控制是一项挑战。在本研究中，基于高效液相色谱（HPLC）、多角度激光散射、粘度计和折射率检测器（HPSEC-MALLS-Vis-RID）的糖谱分析以及傅里叶变换红外光谱（FT-IR）被用于区分九种不同褐藻（BA1-9）中的多糖。结果表明，BSPs由β-D-葡聚糖和β-1,3-1,4-葡聚糖键组成。BSPs的分子量、回转半径和特性粘度分别为1.718×10 Da至6.630×10 Da、30.2 nm至51.5 nm以及360.99 mL/g至865.52 mL/g。此外，BSPs的α值在0.635至0.971范围内，表明其为刚性棒状链构象。BSPs的抗氧化活性对DPPH（1,1-二苯基-2-苦基肼）和ABTS（2, 2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸）自由基表现出显著的清除活性，这表明使用BSPs可能是一种潜在的抗氧化剂补充方法。因此，本研究为BSPs的结构-功能关系提供了见解，这将有助于提高源自海藻的多糖质量。
