Unit of Orthodontics, University Dental Clinic, University of Murcia, Murcia, Spain.
Department of Child Integrated Dentistry, Faculty of Medicine-Dentistry, University of Murcia, Murcia, Spain.
Sci Rep. 2021 Jan 12;11(1):834. doi: 10.1038/s41598-020-80844-7.
To evaluate the effectiveness of a calcium silicate/phosphate fluoridated tooth paste and a serum compared with a toothpaste containing hydroxyapatite on protecting the enamel after interproximal reduction against demineralization. 3 sets of eleven incisors were created. The teeth underwent interproximal enamel reduction (IER) of 0.5 mm. Each set was allocated to one of three groups: (1) Brushing without toothpaste (control group); (2) Vitis toothpaste + Remin Pro; (3) Regenerate toothpaste + Regenerate Serum. The agents were applied three times a day and specimens subjected to demineralization cycles for 30 days. The weight percentages of calcium (Ca) and phosphorous (P) were quantified by X-ray microfluorescence spectroscopy. Surface microhardness measurements and electron scanning microscopy (SEM) observations were made. Ca data and the Ca/P ratio were significantly higher in Group 3 than the other groups (p < 0.017), while P was significantly lower in Group 3 (p < 0.017). No significant differences were found between Groups 1 and 2 (p > 0.017). Group 3 showed significantly higher microhardness values (p < 0.05) than Group 1. No significant differences were found for other comparisons between groups (p < 0.05). SEM images showed less demineralization in Group 3. The application of a calcium silicate/phosphate fluoridated tooth paste (Regenerate advance) and a dual serum (Regenerate advance enamel serum) protect the enamel with interproximal reduction against demineralization. Therefore, this treatment could be used to prevent the dissolution of hydroxyapatite after IER.
评价硅酸钙/磷酸盐氟化牙膏和血清在预防邻面去矿化后釉质脱矿方面的效果，并与含羟磷灰石的牙膏进行比较。创建了 3 组 11 颗切牙。牙齿经历了 0.5mm 的邻面釉质减少（IER）。每组分为三组之一：（1）不使用牙膏刷牙（对照组）；（2）Vitis 牙膏+Remin Pro；（3）Regenerate 牙膏+Regenerate Serum。每天应用 3 次，标本在 30 天内进行脱矿循环。通过 X 射线微荧光光谱法定量测定钙（Ca）和磷（P）的重量百分比。进行表面显微硬度测量和电子扫描显微镜（SEM）观察。与其他组相比，第 3 组的 Ca 数据和 Ca/P 比值明显更高（p<0.017），而第 3 组的 P 明显更低（p<0.017）。第 1 组和第 2 组之间没有发现显著差异（p>0.017）。第 3 组的显微硬度值明显高于第 1 组（p<0.05）。组间其他比较无显著差异（p<0.05）。SEM 图像显示第 3 组脱矿程度较低。硅酸钙/磷酸盐氟化牙膏（Regenerate advance）和双血清（Regenerate advance 牙釉质血清）的应用可预防邻面减少后釉质脱矿。因此，这种治疗方法可用于预防 IER 后羟磷灰石的溶解。
