Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands.
Angew Chem Int Ed Engl. 2021 Mar 29;60(14):7612-7616. doi: 10.1002/anie.202016244. Epub 2021 Feb 26.
Biological processes rely on transient interactions that govern assembly of biomolecules into higher order, multi-component systems. A synthetic platform for the dynamic assembly of multicomponent complexes would provide novel entries to study and modulate the assembly of artificial systems into higher order topologies. Here, we establish a hybrid DNA origami-based approach as an assembly platform that enables dynamic templating of supramolecular architectures. It entails the site-selective recruitment of supramolecular polymers to the platform with preservation of the intrinsic dynamics and reversibility of the assembly process. The composition of the supramolecular assembly on the platform can be tuned dynamically, allowing for monomer rearrangement and inclusion of molecular cargo. This work should aid the study of supramolecular structures in their native environment in real-time and incites new strategies for controlled multicomponent self-assembly of synthetic building blocks.
生物过程依赖于瞬时相互作用，这些相互作用控制着生物分子组装成更高阶的多组分系统。用于动态组装多组分复合物的合成平台将为研究和调节人工系统组装成更高阶拓扑结构提供新的途径。在这里，我们建立了一种混合 DNA 折纸的方法作为一个组装平台，使超分子结构的动态模板化成为可能。它需要通过选择性地招募超分子聚合物到平台上来实现，同时保持组装过程的固有动力学和可逆性。平台上超分子组装的组成可以动态地进行调整，允许单体重排和分子货物的包含。这项工作应该有助于实时研究其天然环境中的超分子结构，并激发用于控制合成构建块的多组分自组装的新策略。
