The Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA.
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA; UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, CA, USA.
Trends Biochem Sci. 2021 May;46(5):406-416. doi: 10.1016/j.tibs.2020.12.002. Epub 2021 Jan 11.
Bacterial microcompartments (MCPs) are extremely large (100-400 nm) and diverse proteinaceous organelles that compartmentalize multistep metabolic pathways, increasing their efficiency and sequestering toxic and/or volatile intermediates. This review highlights recent studies that have expanded our understanding of the diversity, structure, function, and potential biotechnological uses of MCPs. Several new types of MCPs have been identified and characterized revealing new functions and potential new associations with human disease. Recent structural studies of MCP proteins and recombinant MCP shells have provided new insights into MCP assembly and mechanisms and raised new questions about MCP structure. We also discuss recent work on biotechnology applications that use MCP principles to develop nanobioreactors, nanocontainers, and molecular scaffolds.
细菌微室（MCPs）是极其庞大（100-400nm）且多样的蛋白质细胞器，可分隔多步骤代谢途径，提高其效率并隔离有毒和/或挥发性中间产物。这篇综述强调了最近的研究，这些研究扩展了我们对 MCP 的多样性、结构、功能和潜在生物技术用途的理解。已经鉴定和表征了几种新型的 MCP，揭示了新的功能和与人类疾病的潜在新关联。最近对 MCP 蛋白和重组 MCP 壳的结构研究提供了对 MCP 组装和机制的新见解，并提出了关于 MCP 结构的新问题。我们还讨论了最近利用 MCP 原理开发纳米生物反应器、纳米容器和分子支架的生物技术应用工作。
