Shobhaben Pratapbhai Patel School of Pharmacy and Technology Management, SVKM'S NMIMS, V. L. Mehta Road, Vile Parle (W), Mumbai, India.
Stem Cell Rev Rep. 2021 Aug;17(4):1239-1250. doi: 10.1007/s12015-021-10120-2. Epub 2021 Jan 16.
Layer-by-layer deposition of cells, tissues and similar molecules provided by additive manufacturing techniques such as 3D bioprinting offers safe, biocompatible, effective and inert methods for the production of biological structures and biomimetic scaffolds. 3D bioprinting assisted through computer programmes and software develops mutli-modal nano- or micro-particulate systems such as biosensors, dosage forms or delivery systems and other biological scaffolds like pharmaceutical implants, prosthetics, etc. This review article focuses on the implementation of 3D bioprinting techniques in the gene expression, in gene editing or therapy and in delivery of genes. The applications of 3D printing are extensive and include gene therapy, modulation and expression in cancers, tissue engineering, osteogenesis, skin and vascular regeneration. Inclusion of nanotechnology with genomic bioprinting parameters such as gene conjugated or gene encapsulated 3D printed nanostructures may offer new avenues in the future for efficient and controlled treatment and help in overcoming the limitations faced in conventional methods. Moreover, expansion of the benefits from such techniques is advantageous in real-time delivery or in-situ production of nucleic acids into the host cells. Aspects of 3D bioprinting in gene delivery.
通过 3D 生物打印等添加剂制造技术进行的细胞、组织和类似分子的逐层沉积，为生物结构和仿生支架的生产提供了安全、生物相容、有效和惰性的方法。通过计算机程序和软件辅助的 3D 生物打印可开发出多模式纳米或微颗粒系统，如生物传感器、剂型或给药系统以及其他生物支架，如药物植入物、假肢等。本文综述重点介绍了 3D 生物打印技术在基因表达、基因编辑或治疗以及基因传递中的应用。3D 打印的应用非常广泛，包括基因治疗、癌症中的调节和表达、组织工程、成骨、皮肤和血管再生。将纳米技术与基因组生物打印参数（如基因偶联或基因包封的 3D 打印纳米结构）结合使用，可能为未来高效和控制治疗提供新途径，并有助于克服传统方法面临的局限性。此外，此类技术的效益扩展有利于实时递送至宿主细胞内或原位生产核酸。3D 生物打印在基因传递方面的应用。
