Lazurko Caitlin, Khatoon Zohra, Goel Keshav, Sedlakova Veronika, Eren Cimenci Cagla, Ahumada Manuel, Zhang Li, Mah Thien-Fah, Franco Walfre, Suuronen Erik J, Alarcon Emilio I
Division of Cardiac Surgery, University of Ottawa Heart Institute, 40 Ruskin Street, Ottawa K1Y4W7, Canada.
Department of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa K1H8M5, Canada.
ACS Biomater Sci Eng. 2020 Feb 10;6(2):1124-1134. doi: 10.1021/acsbiomaterials.9b01281. Epub 2020 Jan 7.
A novel strategy is needed for treating nonhealing wounds, which is able to simultaneously eradicate pathogenic bacteria and promote tissue regeneration. This would improve patient outcome and reduce the number of lower limb amputations. In this work, we present a multifunctional therapeutic approach able to control bacterial infections, provide a protective barrier to a full-thickness wound, and improve wound healing in a clinically relevant animal model. Our approach uses a nanoengineered antimicrobial nanoparticle for creating a sprayable layer onto the wound bed that prevents bacterial proliferation and also eradicates preformed biofilms. As a protective barrier for the wound, we developed a thermoresponsive collagen-based matrix that has prohealing properties and is able to fill wounds independent of their geometries. Our results indicate that using a combination of the matrix with full-thickness microscopic skin tissue columns synergistically contributed to faster and superior skin regeneration in a nonhealing wound model in diabetic mice.
治疗难愈合伤口需要一种新策略，该策略能够同时根除病原菌并促进组织再生。这将改善患者预后并减少下肢截肢的数量。在这项工作中，我们提出了一种多功能治疗方法，该方法能够控制细菌感染，为全层伤口提供保护屏障，并在临床相关动物模型中改善伤口愈合。我们的方法使用纳米工程抗菌纳米颗粒在伤口床上形成可喷雾层，以防止细菌增殖并根除预先形成的生物膜。作为伤口的保护屏障，我们开发了一种具有促愈合特性且能够独立于伤口几何形状填充伤口的热响应性胶原基基质。我们的结果表明，在糖尿病小鼠的难愈合伤口模型中，将该基质与全层微观皮肤组织柱结合使用可协同促进更快、更优质的皮肤再生。
