Niu Wentao, Huang Hui, Lin Rongcheng, Chen Ching-Hung, Shen Kang-Ning, Hsiao Chung-Der
Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.
Mitochondrial DNA B Resour. 2016 Feb 1;1(1):10-11. doi: 10.1080/23802359.2015.1137796.
In this study, the complete mitogenome sequence of Galaxy Coral, (Cnidaria: Oculinidae), has been sequenced by next-generation sequencing method. The overall base composition of mitogenome is 24.9% for A, 14.0% for C, 24.3% for G and 36.8% for T and has low GC content of 38.3%. The assembled mitogenome, consisting of 18 751 bp, has unique 13 protein-coding genes (PCGs), three transfer RNAs and two ribosomal RNAs genes. The mitogenome has the common mitogenome gene organization and feature of scleractinian coral. Among 13 PCGs, ND5 gene is interrupted by group I intron (12 022 bp). There are 12 genes embedded in ND5 group I intron (tRNA-Leu, ND1, CYTB, ND2, ND6, ATP6, ND4, 12S rRNA, COX3, COX2, ND4L and ND3). The complete mitogenome provides essential and important DNA molecular data for further phylogenetic and evolutionary analysis for stony coral.
在本研究中，通过下一代测序方法对星系珊瑚（刺胞动物门：眼珊瑚科）的完整线粒体基因组序列进行了测序。线粒体基因组的总体碱基组成为：A占24.9%，C占14.0%，G占24.3%，T占36.8%，GC含量较低，为38.3%。组装后的线粒体基因组由18751个碱基对组成，具有独特的13个蛋白质编码基因（PCGs）、三个转运RNA和两个核糖体RNA基因。该线粒体基因组具有石珊瑚常见的线粒体基因组基因组织和特征。在13个PCGs中，ND5基因被I组内含子（12022个碱基对）中断。ND5 I组内含子中嵌入了12个基因（tRNA-亮氨酸、ND1、CYTB、ND2、ND6、ATP6、ND4、12S rRNA、COX3、COX2、ND4L和ND3）。完整的线粒体基因组为石珊瑚的进一步系统发育和进化分析提供了重要的DNA分子数据。
