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低覆盖度基因组测序获得了睡衣海蛞蝓(软体动物门:多彩海牛科)的完整线粒体基因组。

Low-coverage genome sequencing yields the complete mitogenome of Pyjama Slug, (Mollusca: Chromodorididae).

作者信息

Xiang Peng, Lin Mao, Zhao Liyuan, Shen Kang-Ning, Hsiao Chung-Der

机构信息

Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China.

Centre of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan.

出版信息

Mitochondrial DNA B Resour. 2016 Feb 1;1(1):94-95. doi: 10.1080/23802359.2015.1137841.

Abstract

In this study, the complete mitogenome sequence of Pyjama Slug, (Mollusca: Chromodorididae), has been decoded for the first time by low-coverage genome-sequencing method. The overall base composition of mitogenome is 30.6% for A, 14.4% for C, 17.8% for G and 37.2% for T and has low GC content of 32.2%. The assembled mitogenome, consisting of 14 259 bp, has unique 13 protein-coding genes (PCGs), 22 transfer RNAs and two ribosomal RNAs genes. The mitogenome has the common mitogenome gene organization and feature of Nudipleura (a clade of sea slugs and sea snails). The complete mitogenome provides essential and important DNA molecular data for further evolutionary analysis for sea slugs and sea snails.

摘要

在本研究中,首次通过低覆盖度基因组测序方法解码了睡衣海蛞蝓(软体动物:多彩海牛科)的完整线粒体基因组序列。线粒体基因组的总体碱基组成为:A占30.6%,C占14.4%,G占17.8%,T占37.2%,GC含量较低,为32.2%。组装后的线粒体基因组由14259个碱基对组成,具有独特的13个蛋白质编码基因(PCGs)、22个转运RNA和两个核糖体RNA基因。该线粒体基因组具有裸鳃亚目(海蛞蝓和海螺的一个分支)常见的线粒体基因组基因组织和特征。完整的线粒体基因组为海蛞蝓和海螺的进一步进化分析提供了重要的DNA分子数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abe2/7800696/6f4fe7b6e8a4/TMDN_A_1137841_F0001_B.jpg

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