• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

RNAi 介导的 TaCSN5 的稳定沉默赋予了小麦条锈病菌广谱抗性。

RNAi-mediated stable silencing of TaCSN5 confers broad-spectrum resistance to Puccinia striiformis f. sp. tritici.

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling, China.

出版信息

Mol Plant Pathol. 2021 Apr;22(4):410-421. doi: 10.1111/mpp.13034. Epub 2021 Jan 24.

DOI:10.1111/mpp.13034
PMID:33486803
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7938628/
Abstract

The constitutive photomorphogenesis 9 (COP9) signalosome (CSN) is a versatile regulator of plant growth, development, and response to diverse pathogens. However, little research has been done to understand the function of those CSN genes in broad-spectrum resistance to pathogens. In this study, we found that the transcript levels of wheat TaCSN5 were induced in response to inoculation with Puccinia striiformis f. sp. tritici (Pst) and treatment with salicylic acid (SA). Overexpression of TaCSN5 in Arabidopsis resulted in increased susceptibility to Pseudomonas syringae pv. tomato DC3000 infection accompanied by down-regulation of AtPR1 expression. Overexpression of TaCSN5 in wheat lines significantly increased susceptibility to Pst accompanied by decreased SA accumulation, whereas TaCSN5-RNAi wheat lines exhibited opposite trends. Moreover, we found that TaCSN5 negatively regulated TaG3NPR1 genes involved in the SA signalling pathway. In addition, TaCSN5-RNAi lines showed increased resistance to multiple races of Pst. Taken together, we demonstrate that TaCSN5 contributes to negative regulation of wheat resistance to Pst in an SA-dependent manner.

摘要

组成型光形态建成 9（COP9）信号体（CSN）是一种多功能的植物生长、发育和对各种病原体反应的调节剂。然而，对于那些 CSN 基因在广谱抗病性中的功能，研究甚少。在本研究中，我们发现小麦 TaCSN5 的转录水平在接种小麦条锈菌（Pst）和水杨酸（SA）处理后被诱导。在拟南芥中过表达 TaCSN5 会导致对丁香假单胞菌 pv.番茄 DC3000 感染的敏感性增加，同时伴随 AtPR1 表达的下调。在小麦品系中过表达 TaCSN5 会显著增加对 Pst 的敏感性，同时 SA 积累减少，而 TaCSN5-RNAi 小麦系则表现出相反的趋势。此外，我们发现 TaCSN5 负调控参与 SA 信号通路的 TaG3NPR1 基因。此外，TaCSN5-RNAi 系对多个 Pst 菌系表现出增强的抗性。综上所述，我们证明 TaCSN5 通过依赖 SA 的方式负调控小麦对 Pst 的抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/6352ceab65f9/MPP-22-410-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/7627db577fb5/MPP-22-410-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/0ffadf16dc3f/MPP-22-410-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/677679e0301b/MPP-22-410-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/fee383af69a2/MPP-22-410-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/74bedacd8533/MPP-22-410-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/abd4cfe7f652/MPP-22-410-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/6ce0ff5e9bbf/MPP-22-410-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/6352ceab65f9/MPP-22-410-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/7627db577fb5/MPP-22-410-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/0ffadf16dc3f/MPP-22-410-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/677679e0301b/MPP-22-410-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/fee383af69a2/MPP-22-410-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/74bedacd8533/MPP-22-410-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/abd4cfe7f652/MPP-22-410-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/6ce0ff5e9bbf/MPP-22-410-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/903a/7938628/6352ceab65f9/MPP-22-410-g007.jpg

相似文献

1
RNAi-mediated stable silencing of TaCSN5 confers broad-spectrum resistance to Puccinia striiformis f. sp. tritici.RNAi 介导的 TaCSN5 的稳定沉默赋予了小麦条锈病菌广谱抗性。
Mol Plant Pathol. 2021 Apr;22(4):410-421. doi: 10.1111/mpp.13034. Epub 2021 Jan 24.
2
, a Yellow Stripe-Like Transporter Gene, Is Required for Wheat Resistance to f. sp. .一个类黄条纹转运蛋白基因，对小麦抗 f. sp. 是必需的。
Genes (Basel). 2020 Dec 3;11(12):1452. doi: 10.3390/genes11121452.
3
The RLK protein TaCRK10 activates wheat high-temperature seedling-plant resistance to stripe rust through interacting with TaH2A.1.RLK 蛋白 TaCRK10 通过与 TaH2A.1 互作激活小麦抗条锈高温成株抗性。
Plant J. 2021 Dec;108(5):1241-1255. doi: 10.1111/tpj.15513. Epub 2021 Oct 7.
4
TaClpS1, negatively regulates wheat resistance against Puccinia striiformis f. sp. tritici.TaClpS1 负调控小麦对条锈病菌的抗性。
BMC Plant Biol. 2020 Dec 10;20(1):555. doi: 10.1186/s12870-020-02762-0.
5
TaADF7, an actin-depolymerizing factor, contributes to wheat resistance against Puccinia striiformis f. sp. tritici.TaADF7是一种肌动蛋白解聚因子，有助于小麦对条锈菌的抗性。
Plant J. 2014 Apr;78(1):16-30. doi: 10.1111/tpj.12457. Epub 2014 Mar 12.
6
A Putative Effector Pst-18220, from f. sp. , Participates in Rust Pathogenicity and Plant Defense Suppression.来自 f. sp. 的一个推定效应物 Pst-18220 参与锈病致病性和植物防御抑制。
Biomolecules. 2024 Aug 31;14(9):1092. doi: 10.3390/biom14091092.
7
A wheat COP9 subunit 5-like gene is negatively involved in host response to leaf rust.一个小麦COP9亚基5类似基因在宿主对叶锈病的反应中起负向作用。
Mol Plant Pathol. 2017 Jan;18(1):125-133. doi: 10.1111/mpp.12467. Epub 2016 Oct 4.
8
, An AP2/ERF Transcription Factor, Is Positively Involved in Wheat Resistance to f. sp. .Ta1, 一个 AP2/ERF 转录因子，正向参与小麦对 f. sp.. 的抗性。
Int J Mol Sci. 2021 Feb 19;22(4):2080. doi: 10.3390/ijms22042080.
9
Isocitrate lyase promotes Puccinia striiformis f. sp. tritici susceptibility in wheat (Triticum aestivum) by suppressing accumulation of glyoxylate cycle intermediates.异柠檬酸裂解酶通过抑制乙醛酸循环中间产物的积累促进小麦条锈菌（Puccinia striiformis f. sp. tritici）对小麦的易感性。
Plant J. 2024 Aug;119(4):2033-2044. doi: 10.1111/tpj.16908. Epub 2024 Jul 1.
10
TaCAP1 Interacts with TaLHCB1s and Positively Regulates Wheat Resistance Against Stripe Rust.TaCAP1 与 TaLHCB1s 互作并正向调控小麦条锈病抗性。
Phytopathology. 2024 Jul;114(7):1646-1656. doi: 10.1094/PHYTO-09-23-0342-R. Epub 2024 Jul 5.

引用本文的文献

1
The E3 ubiquitin ligase SlATL2 suppresses tomato immunity by promoting SlCSN5a degradation during pv. DC3000 infection.E3泛素连接酶SlATL2在丁香假单胞菌番茄致病变种DC3000感染期间通过促进SlCSN5a降解来抑制番茄免疫。
Hortic Res. 2025 Mar 10;12(6):uhaf078. doi: 10.1093/hr/uhaf078. eCollection 2025 Jun.
2
The Silencing of the Gene Enhanced the Resistance of Potato Plants to the Phytotoxin Thaxtomin A.该基因的沉默增强了马铃薯植株对植物毒素thaxtomin A的抗性。
Int J Mol Sci. 2025 Feb 6;26(3):1361. doi: 10.3390/ijms26031361.
3
Ubiquitination of OsCSN5 by OsPUB45 activates immunity by modulating the OsCUL3a-OsNPR1 module.

本文引用的文献

1
A new mode of NPR1 action via an NB-ARC-NPR1 fusion protein negatively regulates the defence response in wheat to stem rust pathogen.通过一种NB-ARC-NPR1融合蛋白产生的NPR1作用新模式对小麦抗秆锈病病原体的防御反应起负调控作用。
New Phytol. 2020 Nov;228(3):959-972. doi: 10.1111/nph.16748. Epub 2020 Aug 26.
2
COP9 signalosome: Discovery, conservation, activity, and function.COP9 信号体：发现、保守性、活性和功能。
J Integr Plant Biol. 2020 Jan;62(1):90-103. doi: 10.1111/jipb.12903.
3
An efficient and reproducible -mediated transformation method for hexaploid wheat ( L.).
OsPUB45对OsCSN5进行泛素化修饰，通过调节OsCUL3a-OsNPR1模块激活免疫反应。
Sci Adv. 2025 Jan 3;11(1):eadr2441. doi: 10.1126/sciadv.adr2441.
4
All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants.条条大路通罗马：植物工程抗病之路
Adv Sci (Weinh). 2025 Feb;12(5):e2412223. doi: 10.1002/advs.202412223. Epub 2024 Dec 18.
5
The Calcium-Dependent Protein Kinase TaCDPK7 Positively Regulates Wheat Resistance to f. sp. .钙依赖型蛋白激酶 TaCDPK7 正向调控小麦对禾谷镰刀菌的抗性。
Int J Mol Sci. 2024 Jan 15;25(2):1048. doi: 10.3390/ijms25021048.
6
Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.RNA 干扰在植物功能基因组学中的进展：揭示特性、机制和未来方向。
Appl Biochem Biotechnol. 2024 Sep;196(9):5681-5710. doi: 10.1007/s12010-023-04850-x. Epub 2024 Jan 4.
7
The Wheat Annexin Plays Positive Roles in Plant Disease Resistance by Regulating the Accumulation of Reactive Oxygen Species and Callose.小麦 annexin 通过调节活性氧和胼胝质的积累正向调控植物抗病性。
Int J Mol Sci. 2023 Nov 16;24(22):16381. doi: 10.3390/ijms242216381.
8
A candidate effector protein PstCFEM1 contributes to virulence of stripe rust fungus and impairs wheat immunity.候选效应蛋白PstCFEM1有助于条锈病菌的致病性并削弱小麦的免疫力。
Stress Biol. 2022 Apr 8;2(1):21. doi: 10.1007/s44154-022-00042-5.
9
Transcriptome-Wide Integrated Analysis of the Gene in Controlling Ginsenoside Biosynthesis in .转录组范围的基因综合分析在调控人参皂苷生物合成中的作用 。 你提供的原文似乎不太完整，“in.”后面应该还有具体内容。
Plants (Basel). 2023 May 15;12(10):1980. doi: 10.3390/plants12101980.
10
Small RNA-based plant protection against diseases.基于小RNA的植物病害防治
Front Plant Sci. 2022 Aug 18;13:951097. doi: 10.3389/fpls.2022.951097. eCollection 2022.
一种用于六倍体小麦（L.）的高效且可重复的介导转化方法。
Plant Methods. 2019 Oct 26;15:121. doi: 10.1186/s13007-019-0503-z. eCollection 2019.
4
Engineering Broad-Spectrum Bacterial Blight Resistance by Simultaneously Disrupting Variable TALE-Binding Elements of Multiple Susceptibility Genes in Rice.通过同时破坏水稻多个感病基因中可变 TALE 结合元件来工程广谱细菌性条斑病抗性。
Mol Plant. 2019 Nov 4;12(11):1434-1446. doi: 10.1016/j.molp.2019.08.006. Epub 2019 Sep 4.
5
TaCIPK10 interacts with and phosphorylates TaNH2 to activate wheat defense responses to stripe rust.TaCIPK10 与 TaNH2 相互作用并使其磷酸化，从而激活小麦对条锈病的防御反应。
Plant Biotechnol J. 2019 May;17(5):956-968. doi: 10.1111/pbi.13031. Epub 2018 Dec 5.
6
Transcription Factors Associated With -Mediated Acquired Resistance in Barley Are Potential Resources to Improve Wheat Resistance to .与大麦中介导的获得性抗性相关的转录因子是提高小麦对……抗性的潜在资源。
Front Plant Sci. 2018 Oct 17;9:1486. doi: 10.3389/fpls.2018.01486. eCollection 2018.
7
Comparative genome-wide mapping versus extreme pool-genotyping and development of diagnostic SNP markers linked to QTL for adult plant resistance to stripe rust in common wheat.比较全基因组图谱与极端池基因分型以及与普通小麦成株期条锈病抗性 QTL 连锁的诊断 SNP 标记的开发。
Theor Appl Genet. 2018 Aug;131(8):1777-1792. doi: 10.1007/s00122-018-3113-7. Epub 2018 Jun 16.
8
Genome Editing: Targeting Susceptibility Genes for Plant Disease Resistance.基因组编辑：靶向植物疾病抗性的易感基因。
Trends Biotechnol. 2018 Sep;36(9):898-906. doi: 10.1016/j.tibtech.2018.04.005. Epub 2018 May 8.
9
The Wheat Bax Inhibitor-1 Protein Interacts with an Aquaporin TaPIP1 and Enhances Disease Resistance in .小麦Bax抑制剂-1蛋白与水通道蛋白TaPIP1相互作用并增强抗病性。
Front Plant Sci. 2018 Jan 22;9:20. doi: 10.3389/fpls.2018.00020. eCollection 2018.
10
Genome-Wide Identification of Cyclic Nucleotide-Gated Ion Channel Gene Family in Wheat and Functional Analyses of and .小麦中环核苷酸门控离子通道基因家族的全基因组鉴定及TaCNGC15和TaCNGC17的功能分析
Front Plant Sci. 2018 Jan 22;9:18. doi: 10.3389/fpls.2018.00018. eCollection 2018.