Gembloux Agro-Bio Tech, University of Liège, Gembloux 5030, Belgium; National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. Electronic address: http://twitter.com/@SyedShanZaidi.
Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA. Electronic address: http://twitter.com/@SMukhtarlab.
Trends Biotechnol. 2018 Sep;36(9):898-906. doi: 10.1016/j.tibtech.2018.04.005. Epub 2018 May 8.
Plant pathogens pose a major threat to crop productivity. Typically, phytopathogens exploit plants' susceptibility (S) genes to facilitate their proliferation. Disrupting these S genes may interfere with the compatibility between the host and the pathogens and consequently provide broad-spectrum and durable disease resistance. In the past, genetic manipulation of such S genes has been shown to confer disease resistance in various economically important crops. Recent studies have accomplished this task in a transgene-free system using new genome editing tools, including clustered regularly interspaced palindromic repeats (CRISPR). In this Opinion article, we focus on the use of genome editing to target S genes for the development of transgene-free and durable disease-resistant crop varieties.
植物病原体对作物生产力构成重大威胁。通常，植物病原体利用植物的易感性 (S) 基因来促进其增殖。破坏这些 S 基因可能会干扰宿主与病原体之间的兼容性，从而提供广谱和持久的抗病性。过去，对这些 S 基因的遗传操作已被证明可赋予各种经济重要作物的抗病性。最近的研究使用新的基因组编辑工具，包括成簇规律间隔短回文重复序列 (CRISPR)，在无转基因系统中完成了这项任务。在这篇观点文章中，我们重点介绍了使用基因组编辑来靶向 S 基因，以开发无转基因和持久抗病的作物品种。
