Razali Kahina, Kaidi Rachid, Abdelli Amine, Menoueri Mohamed Nabil, Ait-Oudhia Khatima
Laboratory of Animal Reproduction Biotechnologies, Blida, Algeria.
Department of Veterinary Sciences, Institute of Veterinary Sciences, Université Saad Dahlab de Blida 1, Blida, Algeria.
Vet World. 2020 Dec;13(12):2806-2814. doi: 10.14202/vetworld.2020.2806-2814. Epub 2020 Dec 30.
Knowledge of potentially pathogenic bacteria presents in the oral cavity of dogs and cats may be helpful in determining appropriate treatment for infected bite wounds. About 120.000 people are exposed to dog and cat bites every year in Algeria, but little is known about the dog and cat oral flora causing bite wound complications. The purpose of this study was to identify potential zoonotic bacteria from oral cavity of dogs and cats and to determine their susceptibility to antibiotics to contribute to the treatment of bite wound infection.
Oral swabs from 100 stray dogs and 100 stray cats were collected and cultured in several media: Chocolate agar, MacConkey agar, and Mannitol Salt Agar. Bacterial isolates were identified using several commercial kits of the analytical profile index and tested for antibiotic susceptibility by disk diffusion method.
Overall, 185/200 (92.5%) dogs and cats carried zoonotic bacteria in their mouths, of which 55.13% (102/185) had at least two bacterial pathogens. 374 pathogenic strains belonging to 15 genera were isolated: Eleven were Gram-negative (, , , , , , , and ) and four were Gram-positive (, , and , ). Fifty-one strains of were isolated from 44 carriers of (21 , 21 , and 9 spp.). strains were tested for antibiotic resistance. Resistance to at least one drug was observed in 8 (15.68%) of isolates and two strains (3.92%) were found to be multidrug-resistant (to two or more drugs). Erythromycin, penicillin, and ampicillin were the antimicrobials to which the isolates showed greater resistance (7.84%, 5.88%, and 3.92%, respectively).
To the best of our knowledge, this study is the first in Algeria to detect potential human pathogenic bacteria in the oral cavity of dogs and cats. It reveals that these animals have multiple zoonotic bacteria in their mouths including species, which may be multidrug-resistant.
了解存在于犬猫口腔中的潜在致病细菌，可能有助于确定对感染咬伤伤口的适当治疗方法。在阿尔及利亚，每年约有12万人遭受犬猫咬伤，但对于导致咬伤伤口并发症的犬猫口腔菌群了解甚少。本研究的目的是从犬猫口腔中鉴定潜在的人畜共患病细菌，并确定它们对抗生素的敏感性，以有助于治疗咬伤伤口感染。
收集100只流浪狗和100只流浪猫的口腔拭子，并在几种培养基中培养：巧克力琼脂、麦康凯琼脂和甘露醇盐琼脂。使用几种分析谱指数商业试剂盒鉴定细菌分离株，并通过纸片扩散法测试其抗生素敏感性。
总体而言，185/200（92.5%）的犬猫口腔中携带人畜共患病细菌，其中55.13%（102/185）至少有两种细菌病原体。分离出属于15个属的374株致病菌株：11株为革兰氏阴性菌（、、、、、、、和），4株为革兰氏阳性菌（、、和）。从44例携带者（21例、21例和9例 spp.）中分离出51株。对菌株进行了抗生素耐药性测试。在所分离的菌株中，8株（15.68%）对至少一种药物耐药，2株（3.92%）被发现对多种药物耐药（对两种或更多种药物）。红霉素、青霉素和氨苄青霉素是分离株显示出更高耐药性的抗菌药物（分别为7.84%、5.88%和3.92%）。
据我们所知，本研究是阿尔及利亚首次检测犬猫口腔中潜在的人类致病细菌。研究表明，这些动物口腔中存在多种人畜共患病细菌，包括可能对多种药物耐药的物种。
