Ho-Terry L, Terry G M, Londesborough P, Rees K R, Wielaard F, Denissen A
Department of Chemical Pathology, University College and Middlesex Hospital Medical School, London, England.
J Med Virol. 1988 Feb;24(2):175-82. doi: 10.1002/jmv.1890240206.
The efficacy of nucleic acid hybridization for the diagnosis of rubella infection in experimental and clinical materials was compared with immunoblot and virus isolation techniques. Our results showed that nucleic acid hybridization is specific and rapid but gives false-negative results when compared with conventional virus isolation in some experimental although not in clinical materials so far examined. For this reason, a failure to demonstrate rubella virus in fetal specimens by this method alone cannot yet be taken as a sole criterion for ruling out fetal rubella infection.
将核酸杂交技术用于实验材料和临床材料中风疹感染诊断的效果，与免疫印迹法和病毒分离技术进行了比较。我们的结果表明，核酸杂交具有特异性且快速，但在一些实验材料中，与传统病毒分离法相比会出现假阴性结果，不过在目前检测的临床材料中尚未出现这种情况。因此，仅通过这种方法未能在胎儿标本中检测到风疹病毒，尚不能作为排除胎儿风疹感染的唯一标准。
