Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, Suita, Osaka, Japan.
Arch Oral Biol. 2021 Apr;124:105056. doi: 10.1016/j.archoralbio.2021.105056. Epub 2021 Jan 23.
Citrus unshiu has been shown to exhibit antimicrobial effects against citrus diseases. In the present study, C. unshiu was divided into flavedo, albedo, fruits, and leaves; the inhibitory effects of these extracts on Streptococcus mutans, a major pathogen of dental caries, were investigated.
C. unshiu specimens were separated into flavedo, albedo, fruits, and leaves. First, pH values and polyphenol amounts in Citrus extracts were measured. In addition, Citrus extract was added to the bacterial suspensions of S. mutans MT8148, and inhibitory effects of C. unshiu extracts on MT8148 for antimicrobial activity, bacterial growth, and biofilm formation were analyzed. These assays were also performed using C. sinensis extracts.
Among these extracts, albedo exhibited a pH value closest to neutral, while the fruits exhibited the most acidic pH value; the pH values significantly differed between these extracts (P < 0.05). In addition, the amounts of polyphenols were significantly higher in albedo than in other extracts (P < 0.001). All extracts showed inhibitory effects on MT8148 for antimicrobial activity, bacterial growth and biofilm formation. These inhibitory effects were significantly stronger in flavedo, albedo, and fruits, compared with leaves (P < 0.05). Furthermore, extracts of Citrus sinensis also showed inhibitory effects on S. mutans, although these effects were weaker than the effects of C. unshiu.
These results suggest that extracts from C. unshiu fruits exhibit inhibitory effects on S. mutans, among which albedo may be especially useful for dental caries prevention due to its neutral pH and abundant polyphenols, in addition to its inhibitory effects.
已证实柚子具有抗柑橘疾病的抗菌作用。在本研究中，将柚子分为果皮、白皮、果实和叶片；研究了这些提取物对变形链球菌（龋齿的主要病原体）的抑制作用。
将柚子标本分为果皮、白皮、果实和叶片。首先，测量了陈皮提取物的 pH 值和多酚含量。此外，将陈皮提取物添加到变形链球菌 MT8148 的细菌悬浮液中，分析了陈皮提取物对 MT8148 的抗菌活性、细菌生长和生物膜形成的抑制作用。还使用了 C. sinensis 提取物进行了这些测定。
在这些提取物中，白皮的 pH 值最接近中性，而果实的 pH 值最低；这些提取物的 pH 值差异显著（P < 0.05）。此外，白皮中的多酚含量明显高于其他提取物（P < 0.001）。所有提取物均显示出对 MT8148 的抗菌活性、细菌生长和生物膜形成的抑制作用。与叶片相比，白皮、白皮和果实的抑制作用明显更强（P < 0.05）。此外，陈皮提取物也对变形链球菌表现出抑制作用，尽管其作用弱于柚子。
这些结果表明，柚子果实提取物对变形链球菌具有抑制作用，其中白皮由于其中性 pH 值和丰富的多酚含量以及其抑制作用，可能特别有助于预防龋齿。
