Chiu-Lam Andreina, Staples Edward, Pepine Carl J, Rinaldi Carlos
Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.
Thoracic Surgery, University of Florida, Gainesville, FL 32611, USA.
Sci Adv. 2021 Jan 8;7(2). doi: 10.1126/sciadv.abe3005. Print 2021 Jan.
Nanowarming of cryopreserved organs perfused with magnetic cryopreservation agents (mCPAs) could increase donor organ utilization by extending preservation time and avoiding damage caused by slow and nonuniform rewarming. Here, we report formulation of an mCPA containing superparamagnetic iron oxide nanoparticles (SPIONs) that are stable against aggregation in the cryopreservation agent VS55 before and after vitrification and nanowarming and that achieve high-temperature rise rates of up to 321°C/min under an alternating magnetic field. These SPIONs and mCPAs have low cytotoxicity against primary cardiomyocytes. We demonstrate successful perfusion of whole rat hearts with the mCPA and removal using Custodiol HTK solution, even after vitrification, cryostorage in liquid nitrogen for 1 week, and nanowarming under an alternating magnetic field. Quantification of SPIONs in the hearts using magnetic particle imaging demonstrates that the formulated mCPAs are suitable for perfusion, vitrification, and nanowarming of whole organs with minimal residual iron in tissues.
用磁性低温保护剂（mCPA）灌注的低温保存器官的纳米升温可以通过延长保存时间和避免缓慢且不均匀复温造成的损伤来提高供体器官的利用率。在此，我们报告了一种含有超顺磁性氧化铁纳米颗粒（SPIONs）的mCPA的配方，该纳米颗粒在玻璃化和纳米升温前后在低温保护剂VS55中均能稳定抗聚集，并且在交变磁场下可实现高达321°C/分钟的高温升温速率。这些SPIONs和mCPA对原代心肌细胞具有低细胞毒性。我们证明了即使在玻璃化、液氮中冷冻保存1周以及交变磁场下纳米升温后，mCPA仍能成功灌注全大鼠心脏并能用Custodiol HTK溶液去除。使用磁颗粒成像对心脏中的SPIONs进行定量分析表明，所配制的mCPA适用于全器官的灌注、玻璃化和纳米升温，且组织中残留铁最少。
