Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Textile Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Int J Biol Macromol. 2021 Mar 31;174:278-288. doi: 10.1016/j.ijbiomac.2021.01.158. Epub 2021 Jan 29.
In liver tissue engineering, improving the ability of the scaffold to increase the tendency of cells to grow and proliferate is very important. In this study, new methods for modifying the surface of Polycaprolactone (PCL)/Chitosan (Cs) nanofiber for use in liver tissue engineering have been proposed. Galactosylation of chitosan was performed in three ways. According to the FE-SEM, FTIR, NMR and DSC analysis, presence of galactose in uniform nanofibers confirmed and led to a decrease in crystallinity. The hydrophobicity of the scaffolds by contact angle showed that the scaffold with galactosylated after electrospinning, had the highest contact angle of 82.22 ± 2° compared to raw scaffold with 98.52 ± 4°. According to the results of degradation in PBS, the highest rate of degradation was observed in scaffolds that were galactosylated after electrospinning. By culturing HepG2 cells on and based on the results of SEM and MTT analysis, found that the presence of galactose in the scaffolds significantly increased cell growth and proliferation without any toxicity. The immersion method shows a greater ability to improve the growth of liver cells. Also, using in-situ way due to the roughness created in this method may lead to better results especially for in-vivo tests.
在肝脏组织工程中，提高支架增加细胞生长和增殖倾向的能力非常重要。在这项研究中，提出了用于肝脏组织工程的聚己内酯（PCL）/壳聚糖（Cs）纳米纤维表面修饰的新方法。壳聚糖经过三种方法进行了半乳糖基化。根据 FE-SEM、FTIR、NMR 和 DSC 分析，均匀纳米纤维中存在半乳糖得到了证实，并且导致结晶度降低。通过接触角测量了支架的疏水性，结果表明，与原始支架的 98.52±4°相比，经过静电纺丝后半乳糖化的支架具有最高的接触角 82.22±2°。根据 PBS 中的降解结果，发现经过静电纺丝后半乳糖化的支架的降解速度最快。通过在 HepG2 细胞上培养并基于 SEM 和 MTT 分析的结果，发现支架中存在半乳糖显著增加了细胞的生长和增殖，而没有任何毒性。浸泡法显示出增强肝细胞生长的更大能力。此外，由于该方法中产生的粗糙度，原位法可能会产生更好的结果，特别是用于体内测试。
