Skinbetter Science, LLC, Phoenix, Arizona, USA.
J Cosmet Dermatol. 2021 Apr;20(4):1160-1165. doi: 10.1111/jocd.13991. Epub 2021 Feb 18.
Skin damage from visible light predominantly results from exposure to the blue light spectrum (400-500 nm) which generates Reactive Oxygen Species (ROS) causing a cascade of harmful effects to skin. Topical antioxidants reduce the effects of free radical damage caused by environmental exposures. This study evaluated a comprehensive topical antioxidant's ability to inhibit ROS production induced by blue light and cigarette smoke (CS) in human skin.
Two experiments were conducted utilizing human skin (Fitzpatrick Skin Types III and V; N = 3, each). After confirmed reactivity of untreated tissues at 412 nm, 20J/cm , untreated and pretreated (WEL-DS, 2 mg/cm ) skin tissue was exposed to blue light and blue light plus CS and left overnight. A nonfluorescent probe (DCFH-DA) was added to skin and exposed to blue light (412 nm, 20J/cm ) and blue light plus CS. Fluorescent 2',7'-DCF was generated upon enzymatic reduction and subsequent oxidation by ROS.
ROS increased at least tenfold following initial exposure to blue light and blue light plus CS in untreated skin. Pretreatment with WEL-DS decreased ROS in FST III exposed to blue light by 51% and 46% in skin exposed to blue light plus CS vs. untreated skin (both, P < .001). In FST V, pretreatment with WEL-DS decreased ROS exposed to blue light by 54% (P < .001) and 50% in skin exposed to blue light plus CS vs. untreated skin (P < .0001).
WEL-DS demonstrated significant reduction in ROS induced by blue light and blue light in combination with CS compared with untreated, exposed skin.
可见光引起的皮肤损伤主要源于暴露在蓝光光谱（400-500nm）下，该光谱会产生活性氧（ROS），对皮肤造成一系列有害影响。局部抗氧化剂可减少自由基损伤的影响，自由基损伤是由环境暴露引起的。本研究评估了一种综合局部抗氧化剂抑制蓝光和香烟烟雾（CS）在人体皮肤中产生 ROS 的能力。
利用人类皮肤（Fitzpatrick 皮肤类型 III 和 V；N=3，每个）进行了两项实验。在未处理的组织在 412nm 处确认反应性后，用 20J/cm2 的 20J/cm2 的未处理和预处理（WEL-DS，2mg/cm2）皮肤组织暴露于蓝光和蓝光加 CS 下，并过夜。将非荧光探针（DCFH-DA）添加到皮肤中，并暴露于蓝光（412nm，20J/cm2）和蓝光加 CS 下。ROS 酶还原和随后的氧化作用产生荧光 2'，7'-DCF。
未处理的皮肤最初暴露于蓝光和蓝光加 CS 后，ROS 增加了至少十倍。与未处理的皮肤相比，WEL-DS 预处理使 FST III 暴露于蓝光下的 ROS 减少了 51%和 46%，而 FST V 暴露于蓝光加 CS 下的 ROS 减少了 54%（均 P<0.001）。与未处理的皮肤相比，WEL-DS 预处理使暴露于蓝光下的 ROS 减少了 50%（P<0.0001）。
与未处理、暴露的皮肤相比，WEL-DS 显著减少了蓝光和蓝光加 CS 诱导的 ROS。
