Ansari Mohammad Azam, Asiri Sarah Mousa Maadi, Alzohairy Mohammad A, Alomary Mohammad N, Almatroudi Ahmad, Khan Firdos Alam
Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.
Department of Biophysics, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
Pharmaceuticals (Basel). 2021 Feb 9;14(2):139. doi: 10.3390/ph14020139.
The current study demonstrates the synthesis of fatty acids (FAs) capped silver nanoparticles (AgNPs) using aqueous poly-herbal drug Liv52 extract (PLE) as a reducing, dispersing and stabilizing agent. The NPs were characterized by various techniques and used to investigate their potent antibacterial, antibiofilm, antifungal and anticancer activities. GC-MS analysis of PLE shows a total of 37 peaks for a variety of bio-actives compounds. Amongst them, n-hexadecanoic acid (21.95%), linoleic acid (20.45%), oleic acid (18.01%) and stearic acid (13.99%) were found predominately and most likely acted as reducing, stabilizing and encapsulation FAs in LIV-AgNPs formation. FTIR analysis of LIV-AgNPs shows some other functional bio-actives like proteins, sugars and alkenes in the soft PLE corona. The zone of inhibition was 10.0 ± 2.2-18.5 ± 1.0 mm, 10.5 ± 2.5-22.5 ± 1.5 mm and 13.7 ± 1.0-16.5 ± 1.2 against , and , respectively. LIV-AgNPs inhibit biofilm formation in a dose-dependent manner i.e., 54.4% ± 3.1%-10.12% ± 2.3% () 72.7% ± 2.2%-23.3% ± 5.2% () and 85.4% ± 3.3%-25.6% ± 2.2% (), and SEM analysis of treated planktonic cells and their biofilm biomass validated the fitness of LIV-AgNPs in future nanoantibiotics. In addition, as prepared FAs rich PLE capped AgNPs have also exhibited significant ( < 0.05 *) antiproliferative activity against cultured HCT-116 cells. Overall, this is a very first demonstration on employment of FAs rich PLE for the synthesis of highly dispersible, stable and uniform sized AgNPs and their antibacterial, antifungal, antibiofilm and anticancer efficacy.
当前研究表明，使用水性多草药药物Liv52提取物（PLE）作为还原剂、分散剂和稳定剂，可合成脂肪酸（FAs）包覆的银纳米颗粒（AgNPs）。通过多种技术对这些纳米颗粒进行了表征，并用于研究它们强大的抗菌、抗生物膜、抗真菌和抗癌活性。PLE的气相色谱 - 质谱（GC - MS）分析显示，共有37个峰对应多种生物活性化合物。其中，正十六烷酸（21.95%）、亚油酸（20.45%）、油酸（18.01%）和硬脂酸（13.99%）占主导地位，并且很可能在LIV - AgNPs形成过程中充当还原剂、稳定剂和包封脂肪酸。LIV - AgNPs的傅里叶变换红外光谱（FTIR）分析显示，在柔软的PLE冠层中存在一些其他功能性生物活性物质，如蛋白质、糖类和烯烃。对大肠杆菌、金黄色葡萄球菌和白色念珠菌的抑菌圈分别为10.0±2.2 - 18.5±1.0毫米、10.5±2.5 - 22.5±1.5毫米和13.7±1.0 - 16.5±1.2毫米。LIV - AgNPs以剂量依赖方式抑制生物膜形成，即对大肠杆菌为54.4%±3.1% - 10.12%±2.3%，对金黄色葡萄球菌为72.7%±2.2% - 23.3%±5.2%，对白色念珠菌为85.4%±3.3% - 25.6%±2.2%，并且对处理后的浮游细胞及其生物膜生物量的扫描电子显微镜（SEM）分析验证了LIV - AgNPs在未来纳米抗生素方面的适用性。此外，所制备的富含FAs的PLE包覆的AgNPs对培养的HCT - 116细胞也表现出显著的（P < 0.05 *）抗增殖活性。总体而言，这是首次证明使用富含FAs的PLE合成高度分散、稳定且尺寸均匀的AgNPs及其抗菌、抗真菌、抗生物膜和抗癌功效。
