饮食和性别相关的肠道微生物组成和功能谱在 4 个月的减肥干预后的变化。
Diet- and sex-related changes of gut microbiota composition and functional profiles after 4 months of weight loss intervention.
机构信息
Department of Nutrition, Food Sciences and Physiology, University of Navarra, 31008, Pamplona, Spain.
Center for Nutrition Research, University of Navarra, 31008, Pamplona, Spain.
出版信息
Eur J Nutr. 2021 Sep;60(6):3279-3301. doi: 10.1007/s00394-021-02508-0. Epub 2021 Feb 16.
PURPOSE
Obesity has been related to intestinal dysbiosis and the modification of gut microbiota composition by dietary strategies becomes a promising strategy to help manage obesity. The aim of the current study was to evaluate the effect of two weight-loss diets on the composition and functional profile of gut microbiota.
METHODS
55 men and 124 women with BMI > 25 kg/m were randomly assigned to moderately high-protein (MHP) or low-fat (LF) diet. Differences in fecal bacteria abundance (based on 16 s rRNA sequencing) between before and after 4 months of calorie restriction was analyzed using EdgeR tool in MicrobiomeAnalyst platform. Bacterial functional profile was predicted using Tax4Fun and metagenomeSeq analysis. Significant KEGG Orthology (KO) terms were selected for the metabolomic study using chromatography.
RESULTS
After the intervention, MHP-men showed a significant decrease in Negativicutes, Selenomonadales, Dielma and Dielma fastidiosa. LF-men showed a significant increase in Bacilli, Lactobacillales, Christensenellaceae, Peptococcaceae, and Streptococcaceae, Peptococcus, Streptococcus and Christensenella, Duncaniella dubosii_CP039396_93.49%, Roseburia sp_AB744234_98.96% and Alistipes inops_KJ572413_99.57%. MHP-women increased Pasteurellales, Phascolarctobacterium succinatutens, Ruthenibacterium lactatiformans_LR215981_99.55% and decreased in Phascolarctobacterium succinatutens_NR112902_99.56%. Finally, LF-women presented a significant decrease in Bacteroides clarus and Erysipelothrix inopinata_CP060715_84.4%. Surprisingly, no matching bacterial changes were found between these four groups. A total of 42 KO, 10 metabolic pathways and 107 related metabolites related were found implicated in these bacterial changes. Seven metabolites were confirmed in plasma.
CONCLUSION
Weight-loss-related-changes in gut microbiome composition and the functional profile occur in a sex- and diet-related manner, showing that women and men could differentially benefit from the consumption of MHP and LF diets.
TRIAL REGISTRATION
NCT02737267, 10th March 2016 retrospectively registered.
目的
肥胖与肠道菌群失调有关，通过饮食策略改变肠道微生物组成是一种很有前途的帮助控制肥胖的策略。本研究旨在评估两种减肥饮食对肠道微生物组成和功能谱的影响。
方法
将 55 名男性和 124 名 BMI>25kg/m2 的女性随机分配到高蛋白（MHP）或低脂肪（LF）饮食组。使用 MicrobiomeAnalyst 平台中的 EdgeR 工具分析 4 个月热量限制前后粪便细菌丰度（基于 16s rRNA 测序）的差异。使用 Tax4Fun 和 metagenomeSeq 分析预测细菌功能谱。使用色谱法选择显著的 KEGG 同源物（KO）术语进行代谢组学研究。
结果
干预后，MHP 男性的 Negativicutes、Selenomonadales、Dielma 和 Dielma fastidiosa 显著减少。LF 男性的 Bacilli、Lactobacillales、Christensenellaceae、Peptococcaceae 和 Streptococcaceae、Peptococcus、Streptococcus 和 Christensenella、Duncaniella dubosii_CP039396_93.49%、Roseburia sp_AB744234_98.96%和 Alistipes inops_KJ572413_99.57%显著增加。MHP 女性的 Pasteurellales、Phascolarctobacterium succinatutens、Ruthenibacterium lactatiformans_LR215981_99.55%增加，Phascolarctobacterium succinatutens_NR112902_99.56%减少。最后，LF 女性的 Bacteroides clarus 和 Erysipelothrix inopinata_CP060715_84.4%显著减少。令人惊讶的是，这四个组之间没有发现匹配的细菌变化。共发现与这些细菌变化相关的 42 个 KO、10 个代谢途径和 107 种相关代谢物。在血浆中确认了 7 种代谢物。
结论
与体重减轻相关的肠道微生物组成和功能谱的变化以性别和饮食相关的方式发生，表明女性和男性可能会从 MHP 和 LF 饮食的摄入中获得不同的益处。
试验注册
NCT02737267，2016 年 3 月 10 日回顾性注册。
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