Department of Human Genetics, KU Leuven, Leuven, Belgium.
Aix-Marseille Univ, Inserm, CNRS, Institut Paoli-Calmettes, CRCM, Equipe Labellisée LIGUE 2018, Marseille, France.
Sci Rep. 2021 Feb 18;11(1):4083. doi: 10.1038/s41598-021-81697-4.
Exosomal transfers represent an important mode of intercellular communication. Syntenin is a small scaffold protein that, when binding ALIX, can direct endocytosed syndecans and syndecan cargo to budding endosomal membranes, supporting the formation of intraluminal vesicles that compose the source of a major class of exosomes. Syntenin, however, can also support the recycling of these same components to the cell surface. Here, by studying mice and cells with syntenin-knock out, we identify syntenin as part of dedicated machinery that integrates both the production and the uptake of secreted vesicles, supporting viral/exosomal exchanges. This study significantly extends the emerging role of heparan sulfate proteoglycans and syntenin as key components for macromolecular cargo internalization into cells.
外泌体转移代表了细胞间通讯的一种重要模式。连接蛋白（syntenin）是一种小的支架蛋白，当与 ALIX 结合时，可以将内吞的 syndecans 和 syndecan 货物引导到出芽的内体膜上，支持形成组成主要一类外泌体的腔内小泡。然而，连接蛋白也可以支持这些相同成分的再循环到细胞表面。在这里，通过研究 syntenin 敲除的小鼠和细胞，我们确定连接蛋白是专门的机器的一部分，该机器整合了分泌囊泡的产生和摄取，支持病毒/外泌体交换。这项研究显著扩展了肝素硫酸蛋白聚糖和连接蛋白作为细胞内大分子货物内化的关键成分的新兴作用。
