Gupta Kriti, Patel Ruchi, Dias Madara, Ishaque Hina, White Kristopher, Olabisi Ronke
Department of Biomedical Engineering, Rutgers University, Piscataway, NJ, USA.
Graduate School of Biomedical Sciences, Rutgers University, Piscataway, NJ, USA.
Int J Biomater. 2021 Jan 30;2021:6669504. doi: 10.1155/2021/6669504. eCollection 2021.
For many cells used in tissue engineering applications, the scaffolds upon which they are seeded do not entirely mimic their native environment, particularly in the case of excitable tissues. For instance, muscle cells experience contraction and relaxation driven by the electrical input of an action potential. Electroactive materials can also deform in response to electrical input; however, few such materials are currently suitable as cell scaffolds. We previously described the development of poly(ethyelene glycol) diacrylate-poly(acrylic acid) as an electroactive scaffold. Although the scaffold itself supported cell growth and attachment, the voltage (20 V) required to actuate these scaffolds was cytotoxic. Here, we describe the further development of our hydrogels into scaffolds capable of actuation at voltages (5 V) that were not cytotoxic to seeded cells. This study describes the critical next steps towards the first functional electroactive tissue engineering scaffold.
对于组织工程应用中使用的许多细胞而言，接种细胞的支架并不能完全模拟其天然环境，在可兴奋组织的情况下尤其如此。例如，肌肉细胞会在动作电位的电输入驱动下经历收缩和舒张。电活性材料也会响应电输入而发生形变；然而，目前很少有这类材料适合用作细胞支架。我们之前描述了聚乙二醇二丙烯酸酯 - 聚丙烯酸作为一种电活性支架的开发情况。尽管该支架本身能够支持细胞生长和附着，但驱动这些支架所需的电压（20 V）具有细胞毒性。在此，我们描述了将我们的水凝胶进一步开发成能够在对接种细胞无细胞毒性的电压（5 V）下驱动的支架。这项研究描述了迈向首个功能性电活性组织工程支架的关键后续步骤。
