合成酶催化的 CO 固定反应。

Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, CB2 1GA, UK.

Manchester Institute of Biotechnology, Department of Chemistry, University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.

ChemSusChem. 2021 Apr 22;14(8):1781-1804. doi: 10.1002/cssc.202100159. Epub 2021 Mar 10.

In recent years, (de)carboxylases that catalyze reversible (de)carboxylation have been targeted for application as carboxylation catalysts. This has led to the development of proof-of-concept (bio)synthetic CO fixation routes for chemical production. However, further progress towards industrial application has been hampered by the thermodynamic constraint that accompanies fixing CO to organic molecules. In this Review, biocatalytic carboxylation methods are discussed with emphases on the diverse strategies devised to alleviate the inherent thermodynamic constraints and their application in synthetic CO -fixation cascades.

近年来，催化可逆（脱）羧化反应的（脱）羧酶已成为羧化催化剂的应用目标。这促使人们开发了用于化学生产的概念验证（生物）合成 CO 固定途径。然而，将 CO 固定到有机分子所伴随的热力学限制阻碍了进一步向工业应用的发展。本文综述了生物催化羧化方法，重点讨论了设计用于缓解固有热力学限制的各种策略及其在合成 CO 固定级联反应中的应用。