Cairone Francesco, Angiolella Letizia, Bertini Francesca, Iazzetti Antonia, Fabrizi Giancarlo, Petralito Stefania, Cesa Stefania, Simonetti Giovanna
Department Chemistry and Technologies of Drug, University "La Sapienza" of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
Department of Public Health and Infectious Diseases, University "La Sapienza" of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
Foods. 2025 Apr 11;14(8):1332. doi: 10.3390/foods14081332.
L. is acknowledged for its extraordinary phytotherapeutic properties due to the presence of polyphenols, carotenoids, and flavonoids such as β-cryptoxanthin and rutin. These compounds are largely distributed in the skin and flesh of the fruit. In this study, the different parts of persimmons were analyzed (whole fruit, peels, and flesh), aiming at determining total carotenoid and flavonoid content through selective extraction and HPLC-DAD analysis. Obtained by a one-pot double phase extraction, organic and aqueous extracts were submitted to colorimetric analyses and tested for their antifungal activity. Results indicated that carotenoid and flavonoid content varied with sample maturity, and colorimetry proved to be an effective predictor of pigments' composition. The strongest antifungal and antibiofilm activity has been demonstrated for the hydroalcoholic extracts of the unripe whole fruit and flesh. Preliminary results suggest their potential application in preventing infections by inhibiting their establishment. Although further studies are needed, these results open the way to the possible use of the extracts as additives in foods or in the preparation of pharmaceutical formulations for the prevention of infections caused by , helping to reduce the use of synthetic biocidal products.
由于含有多酚、类胡萝卜素以及诸如β-隐黄质和芦丁等黄酮类化合物，L.因其非凡的植物治疗特性而受到认可。这些化合物大量分布于果实的果皮和果肉中。在本研究中，对柿子的不同部分（整个果实、果皮和果肉）进行了分析，旨在通过选择性提取和高效液相色谱-二极管阵列检测（HPLC-DAD）分析来测定总类胡萝卜素和黄酮类化合物的含量。通过一锅双相萃取获得的有机提取物和水提取物进行了比色分析，并测试了它们的抗真菌活性。结果表明，类胡萝卜素和黄酮类化合物的含量随样品成熟度而变化，比色法被证明是色素组成的有效预测指标。未成熟的整个果实和果肉的水醇提取物表现出最强的抗真菌和抗生物膜活性。初步结果表明它们在通过抑制感染的形成来预防感染方面具有潜在应用价值。尽管还需要进一步研究，但这些结果为提取物作为食品添加剂或用于制备预防由 引起的感染的药物制剂开辟了道路，有助于减少合成杀菌产品的使用。
