Abdollahzade Fard Amin, Saboory Ehsan, Tahmazi Yaghob, Rasmi Yousef, Dindarian Sina, Parsamanesh Negin
Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran.
Iran J Basic Med Sci. 2021 Jan;24(1):30-37. doi: 10.22038/ijbms.2020.47479.10930.
This study aimed to assess the impact of orally-administrated thymoquinone (TQ) during pregnancy on litter size, pentylenetetrazol-induced seizure, and body weight in rat offspring.
In this experimental study, 64 pregnant rats were divided into groups according to the doses of TQ (0,10, 40, and 80 mg/kg) and gestational week (GW2 and GW3) of TQ administration. After parturition, the pups were counted, weighed, and assessed for pentylenetetrazol (PTZ)-induced seizure on postnatal days 14 (P14) and 21 (P21).
In GW2 treated rats, TQ 40 mg/kg decreased seizure stages compared with control only on P14 while seizure duration significantly decreased on P14 and P21. On P14, 40 mg/kg TQ increased latency to the first seizure but decreased it on P21. In addition, 40 mg/kg dose decreased body weight (BW) on P1, P14, and P21 compared with 10 mg/kg dose and control groups. The dose of 80 mg/kg led to a complete pregnancy loss. In GW3 treated rats, only 10 mg/kg TQ decreased the seizure stages on P14 and P21. None of the doses had a significant effect on seizure duration and latency. TQ 40 and 80 mg/kg led to a low birth weight while increased BW on P14 and P21. A 50% decrease in litter size was observed in 80 mg/kg treated rats.
Prenatal TQ may have anticonvulsant effects. The effects of TQ on BW of offspring depend on its dose and administration time. Also, a high dose of TQ at GW2 can be severely toxic for pregnancy.
本研究旨在评估孕期口服百里醌（TQ）对大鼠后代的窝仔数、戊四氮诱发惊厥及体重的影响。
在本实验研究中，64只怀孕大鼠根据TQ剂量（0、10、40和80mg/kg）及TQ给药的孕周（妊娠第2周和第3周）分组。分娩后，对幼崽进行计数、称重，并在出生后第14天（P14）和第21天（P21）评估戊四氮（PTZ）诱发惊厥的情况。
在妊娠第2周给药的大鼠中，与对照组相比，40mg/kg TQ仅在P14时降低了惊厥阶段，而在P14和P21时惊厥持续时间显著缩短。在P14时，40mg/kg TQ增加了首次惊厥的潜伏期，但在P21时则缩短了潜伏期。此外，与10mg/kg剂量组和对照组相比，40mg/kg剂量在P1、P14和P21时降低了体重（BW）。80mg/kg剂量导致完全流产。在妊娠第3周给药的大鼠中，只有10mg/kg TQ在P14和P21时降低了惊厥阶段。各剂量对惊厥持续时间和潜伏期均无显著影响。40和80mg/kg TQ导致出生体重低，而在P14和P21时体重增加。在80mg/kg处理的大鼠中观察到窝仔数减少了50%。
产前TQ可能具有抗惊厥作用。TQ对后代体重的影响取决于其剂量和给药时间。此外，妊娠第2周时高剂量的TQ对妊娠具有严重毒性。
