Yu Baozhong, Zheng Xiang, Sun Zejia, Cao Peng, Zhang Jiandong, Wang Wei
Department of Urology, Affiliated Beijing Chaoyang Hospital of Capital Medical University, Beijing, China.
Department of Clinical Medicine, Capital Medical University, Beijing, China.
Front Genet. 2021 Feb 15;12:599952. doi: 10.3389/fgene.2021.599952. eCollection 2021.
The incidences of renal cell carcinoma (RCC) increase in number each year and account for about 2-3% of all malignant tumors. Many patients have metastasis by the time of diagnosis, and their prognosis is poor. Therefore, it is essential that new diagnostic and prognostic markers for kidney cancer are identified. In this study, we assessed the potential of as a diagnostic and prognostic marker for RCC. We analyzed the TCGA and UALCAN databases and found to be highly expressed in ccRCC. In addition, high levels positively correlated with lymphatic metastasis, tumor stage, and histopathological grade. Kaplan-Meier curve analysis showed that expression was related to the prognosis of patients, and high expression indicated a worse overall survival ( = 5.1E-0.7). Receiver operating characteristic curve analysis showed that a combination of expression and histopathological grade improved predictive accuracy (AUC = 0.697; 95%CI: 0.628-0.765, < 0.001). Finally, the relative levels of in ACHN and Caki-1 cells were higher than that of HK-2 cells by western blotting analysis and RT-PCR. Functional tests showed that knocking down expression inhibited migration and invasion . Therefore, is a potential biomarker for the diagnosis and prognosis of RCC patients.
肾细胞癌（RCC）的发病率逐年上升，约占所有恶性肿瘤的2%-3%。许多患者在确诊时已发生转移，预后较差。因此，确定新的肾癌诊断和预后标志物至关重要。在本研究中，我们评估了[具体物质名称未给出]作为RCC诊断和预后标志物的潜力。我们分析了TCGA和UALCAN数据库，发现[具体物质名称未给出]在ccRCC中高表达。此外，高水平的[具体物质名称未给出]与淋巴转移、肿瘤分期和组织病理学分级呈正相关。Kaplan-Meier曲线分析表明，[具体物质名称未给出]的表达与患者预后相关，高表达表明总生存期较差（P = 5.1E - 0.7）。受试者工作特征曲线分析表明，[具体物质名称未给出]表达与组织病理学分级相结合可提高预测准确性（AUC = 0.697；95%CI：0.628 - 0.765，P < 0.001）。最后，通过蛋白质免疫印迹分析和逆转录-聚合酶链反应，ACHN和Caki - 1细胞中[具体物质名称未给出]的相对水平高于HK - 2细胞。功能测试表明，敲低[具体物质名称未给出]表达可抑制迁移和侵袭。因此，[具体物质名称未给出]是RCC患者诊断和预后的潜在生物标志物。
