Lashin Islam, Fouda Amr, Gobouri Adil A, Azab Ehab, Mohammedsaleh Zuhair M, Makharita Rabab R
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt.
Department of Biology, Faculty of Science and Arts, Al-Mandaq Al-Baha University, Al-Baha 1988, Saudi Arabia.
Biomolecules. 2021 Feb 24;11(3):341. doi: 10.3390/biom11030341.
The in vitro callus induction of L. was executed on MS medium supplemented with different concentrations of auxin and cytokinin utilizing petioles and explants of leaves. The highest significant fresh weights from petioles and leaf explants were 4.68 and 5.13 g/jar for the medium supplemented with1.0 mg L BA and 1.0 mg L 2,4-D. The callus extract of the leaves was used for the green synthesis of silver nanoparticles (Ag-NPs). Analytical methods used for Ag-NPs characterization were UV-vis spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and Transmission Electron Microscopy (TEM). Spherical, crystallographic Ag-NPs with sizes ranging from 15 to 60nm were successfully formed. The FT-IR spectra exhibited the role of the metabolites involved in callus extract in reducing and capping Ag-NPs. The biological activities of Ag-NPs were dose-dependent. The MIC value for , , and was 12.5 µg mL, while it was 6.25 µg mL for , and . The highest inhibition of phytopathogenic fungi , , , and was 76.3 ± 3.7, 88.9 ± 4.1, 67.8 ± 2.1, and 76.4 ± 1.0%, respectively at 200 µg mL. Moreover, green synthesized Ag-NPs showed cytotoxic efficacy against cancerous cell lines HepG2, MCF-7 and normal Vero cell line with IC values of 21.76 ± 0.56, 50.19 ± 1.71, and 129.9 ± 0.94 µg mL, respectively.
利用叶柄和叶片外植体，在添加不同浓度生长素和细胞分裂素的MS培养基上对L.进行体外愈伤组织诱导。对于添加1.0 mg/L BA和1.0 mg/L 2,4-D的培养基，叶柄和叶片外植体的最高鲜重分别为4.68和5.13 g/瓶。叶片的愈伤组织提取物用于银纳米颗粒（Ag-NPs）的绿色合成。用于Ag-NPs表征的分析方法有紫外可见光谱、傅里叶变换红外光谱（FT-IR）、X射线衍射（XRD）和透射电子显微镜（TEM）。成功形成了尺寸范围为15至60nm的球形晶体Ag-NPs。FT-IR光谱显示了愈伤组织提取物中参与还原和包覆Ag-NPs的代谢产物的作用。Ag-NPs的生物活性呈剂量依赖性。对于、和，MIC值为12.5 μg/mL，而对于、和，MIC值为6.25 μg/mL。在200 μg/mL时，对植物病原真菌、、和的最高抑制率分别为76.3±3.7%、88.9±4.1%、67.8±2.1%和76.4±1.0%。此外，绿色合成的Ag-NPs对癌细胞系HepG2和MCF-7以及正常Vero细胞系具有细胞毒性作用，IC值分别为21.76±0.56、50.19±1.71和129.9±0.94 μg/mL。
