Greene R T, Walker R L, Nicholson W L, Heidner H W, Levine J F, Burgess E C, Wyand M, Breitschwerdt E B, Berkhoff H A
Department of Companion Animal and Special Species Medicine, North Carolina State University School of Veterinary Medicine, Raleigh 27606.
J Clin Microbiol. 1988 Apr;26(4):648-53. doi: 10.1128/jcm.26.4.648-653.1988.
Immunoblots were used to study the immunoglobulin G response to Borrelia burgdorferi in experimentally and naturally exposed dogs. Adsorption studies confirmed that the antibodies were specific for B. burgdorferi. Experimentally exposed dogs were asymptomatic. Naturally exposed dogs included both asymptomatic animals and animals showing signs compatible with Lyme disease. Naturally exposed dogs were from four geographic regions of the country. No differences were detected between immunoblot patterns of naturally exposed symptomatic or asymptomatic dogs from different areas of the country. The immunoblot patterns obtained with sera from experimentally exposed dogs were different from those obtained with sera from naturally exposed dogs and were characterized by reactivity to fewer and different protein bands. Immunoblot analysis using an OspA-protein-producing Escherichia coli recombinant showed that experimentally exposed dogs produced antibodies to OspA, whereas naturally exposed dogs did not. Modifications of the immune response over time, different routes of antigen presentation, and strain variation are factors postulated to account for the observed differences.
免疫印迹法用于研究实验性暴露和自然暴露犬对伯氏疏螺旋体的免疫球蛋白G反应。吸附研究证实这些抗体对伯氏疏螺旋体具有特异性。实验性暴露的犬没有症状。自然暴露的犬包括无症状动物和表现出与莱姆病相符体征的动物。自然暴露的犬来自该国的四个地理区域。在该国不同地区自然暴露的有症状或无症状犬的免疫印迹模式之间未检测到差异。实验性暴露犬血清获得的免疫印迹模式与自然暴露犬血清获得的不同，其特征是对较少且不同的蛋白条带具有反应性。使用产生OspA蛋白的大肠杆菌重组体进行的免疫印迹分析表明，实验性暴露的犬产生了针对OspA的抗体，而自然暴露的犬则没有。随着时间推移免疫反应的改变、抗原呈递的不同途径以及菌株变异是推测导致观察到的差异的因素。
