Vieira de Morais Daniel, Rosalen Pedro Luiz, Ikegaki Masaharu, de Souza Silva Anna Paula, Massarioli Adna Prado, de Alencar Severino Matias
Center for Nuclear Energy in Agriculture, University of São Paulo, Piracicaba, SP 13416-000, Brazil.
Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, MG 37130-001, Brazil.
Antioxidants (Basel). 2021 Feb 16;10(2):297. doi: 10.3390/antiox10020297.
Brazilian red propolis (BRP) is a natural product widely known for its phenolic composition and strong antioxidant properties. In this study, we used the Box-Behnken Design (BBD) with Surface Response Methodology to optimize the extraction conditions for total phenolic content (TPC) and Trolox equivalent antioxidant capacity(TEAC) of bioactive phenolics from BRP. The extraction time, ethanol/water concentration and temperature, were tested. All variables had significant effects ( ≤ 0.05), with a desirability coefficient of 0.88. Under optimized conditions (90% ethanol at 80 °C for 30 min), the BRP extract showed a TPC of 129.00 ± 2.16 mg GAE/g and a TEAC of 3471.76 ± 53.86 µmol TE/g. Moreover, FRAP and ORAC assays revealed that the optimized BRP extract had 1472.86 ± 72.37 µmol Fe/g and 4339.61 ± 114.65 µmol TE/gof dry weight, respectively. Thirty-two phenolic compounds were tentatively identified by LC-QTOF-ESI-MS/MS, of which thirteen were found for the first time in BRP, including four flavones, one flavanol, two flavanones, two chalcones, and four isoflavonoids. Thus, our results highlight the importance of BRP as a source of a wide variety of phenolic compounds with significant antioxidant properties.
巴西红蜂胶（BRP）是一种天然产物，因其酚类成分和强大的抗氧化性能而广为人知。在本研究中，我们采用Box-Behnken设计（BBD）和表面响应方法来优化从BRP中提取生物活性酚类物质的总酚含量（TPC）和Trolox当量抗氧化能力（TEAC）的提取条件。对提取时间、乙醇/水浓度和温度进行了测试。所有变量均具有显著影响（≤0.05），可取性系数为0.88。在优化条件下（80℃用90%乙醇提取30分钟），BRP提取物的TPC为129.00±2.16毫克没食子酸当量/克，TEAC为3471.76±53.86微摩尔TE/克。此外，FRAP和ORAC分析表明，优化后的BRP提取物干重分别具有1472.86±72.37微摩尔铁/克和4339.61±114.65微摩尔TE/克。通过LC-QTOF-ESI-MS/MS初步鉴定出32种酚类化合物，其中13种是首次在BRP中发现，包括4种黄酮、1种黄烷醇、2种黄烷酮、2种查耳酮和4种异黄酮。因此，我们的结果突出了BRP作为具有显著抗氧化性能的多种酚类化合物来源的重要性。
