Departamento de Química Inorgánica, CINBIO, Universidade de Vigo, 36310 Vigo, Spain.
Centro de Investigación Marina, Universidade de Vigo, 36331 Vigo, Spain.
Molecules. 2021 Feb 23;26(4):1182. doi: 10.3390/molecules26041182.
Macroalgae are sources of bioactive compounds that are interesting from both a chemical and a medical point of view. Although their use in biomedicine has increased significantly in recent years, tests conducted to date have been mostly related to species from temperate latitudes, with the potential application of Antarctic biodiversity being minor. The wide variety of algae species present on Antarctic coastal areas can be a source of new antioxidants. Bearing this in mind, the brown macroalgae (DA) and the red (IC) were selected for the preparation of aqueous extracts with the aim of analyzing their antioxidant activity. This analysis was performed by determining reducing power, total phenolic content, and 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity. Furthermore, both extracts were employed to synthesize gold and silver nanoparticles. The nanomaterials were fully characterized by means of UV-Visible spectroscopy, transmission electron microscopy, Z potential measurements, and Fourier transform infrared spectroscopy, which confirmed the formation of stable, spherical nanoparticles with mean diameters of 13.7 ± 3.1 and 17.5 ± 3.7 nm for Ag@DA and Ag@IC and 12.6 ± 1.9 and 12.3 ± 1.6 nm for Au@DA and Au@IC. Antioxidant assays were performed after the synthesis of the nanomaterials to evaluate their possible synergistic effect with the extracts. The results suggest that polysaccharides and proteins may play a key role in the process of reduction and stabilization. Finally, for the sake of comparison, the results obtained for the Antarctic macroalgae and have also been considered in the present work.
大型藻类是具有生物活性化合物的来源，从化学和医学的角度来看都很有趣。尽管近年来它们在生物医学中的应用有了显著的增加，但迄今为止进行的测试主要与来自温带地区的物种有关，南极生物多样性的潜在应用则较少。南极沿海地区存在的大量藻类物种可以成为新抗氧化剂的来源。考虑到这一点，选择了棕色大型藻类 (DA) 和红色大型藻类 (IC) 来制备水提物，旨在分析它们的抗氧化活性。通过测定还原能力、总酚含量和 2,2-二苯基-1-苦基肼自由基清除活性来进行此分析。此外，还使用这两种提取物来合成金和银纳米粒子。通过紫外-可见光谱、透射电子显微镜、Z 电位测量和傅里叶变换红外光谱对纳米材料进行了全面表征，证实了稳定、球形纳米粒子的形成，其平均直径分别为 13.7 ± 3.1nm 和 17.5 ± 3.7nm 对于 Ag@DA 和 Ag@IC，以及 12.6 ± 1.9nm 和 12.3 ± 1.6nm 对于 Au@DA 和 Au@IC。在合成纳米材料后进行抗氧化测定，以评估它们与提取物的可能协同作用。结果表明，多糖和蛋白质可能在还原和稳定过程中发挥关键作用。最后，为了进行比较，本工作还考虑了南极大型藻类 和 的结果。
