Tazehabadi Mahtab Hassanpour, Algburi Ammar, Popov Igor V, Ermakov Alexey M, Chistyakov Vladimir A, Prazdnova Evgeniya V, Weeks Richard, Chikindas Michael L
Department of Biological Sciences, School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, United States.
Department of Biotechnology, College of Science, University of Diyala, Baqubah, Iraq.
Front Microbiol. 2021 Feb 17;12:615328. doi: 10.3389/fmicb.2021.615328. eCollection 2021.
Salmonellosis is a foodborne infection caused by Domestic poultry species are one of the main reservoirs of , which causes the foodborne infection salmonellosis, and are responsible for many cases of animal-to-human transmission. Keeping backyard chickens is now a growing trend, increasing the frequency of direct contact with the flock and, by consequence, the incidence of infections. KATMIRA1933 and B-1895 are probiotic bacilli that produce the bacteriocins subtilosin A and subtilin, respectively. The antimicrobial activity of the two strains was determined against the reference strain ATCC 10420. The cell-free supernatant of KATMIRA1933 inhibited biofilm formation by subsp. serovar Hadar, subsp. serovar Enteritidis phage type 4, and subsp. serovar Thompson by 51.1, 48.3, and 56.9%, respectively. The cell-free supernatant of B-1895 inhibited the biofilm formation of these strains by 30.4, 28.6, and 35.5%, respectively. These findings suggest that the bacillus strains may have the potential to be used as probiotics and antibiotic alternatives for the control of in poultry. The number of planktonic cells was unaffected by treatment with the cell-free supernatant. A co-culture of the strains with either bacilli showed no signs of growth inhibition, suggesting that it might have been quorum sensing that is affected by the two strains.
沙门氏菌病是一种食源性感染，由家鸡品种引起，家鸡品种是沙门氏菌的主要宿主之一，沙门氏菌会导致食源性感染沙门氏菌病，并导致许多动物传播给人类的病例。现在，饲养后院鸡的趋势越来越明显，这增加了与鸡群直接接触的频率，进而增加了感染的发生率。KATMIRA1933和B - 1895是分别产生细菌素枯草菌素A和枯草菌素的益生菌杆菌。测定了这两种菌株对参考菌株ATCC 10420的抗菌活性。KATMIRA1933的无细胞上清液分别抑制哈达尔亚种、肠炎亚种4型噬菌体和汤普森亚种形成生物膜的能力达51.1%、48.3%和56.9%。B - 1895的无细胞上清液分别抑制这三种菌株形成生物膜的能力达30.4%、28.6%和35.5%。这些发现表明，这些杆菌菌株可能有潜力用作益生菌和抗生素替代品，用于控制家禽中的沙门氏菌。无细胞上清液处理对浮游细胞数量没有影响。这三种菌株与任何一种杆菌的共培养均未显示出生长抑制迹象，这表明可能是群体感应受到了这两种杆菌菌株的影响。
