Hirosue A, Yamamoto K, Shiraki H, Kiyokawa H, Maeda Y, Yoshinari M
Fukuoka Red Cross Blood Center, Japan.
Transfusion. 1988 May-Jun;28(3):261-4. doi: 10.1046/j.1537-2995.1988.28388219156.x.
A method for the preparation of white-cell-poor red cells from 400 ml of blood collected in a quadruple bag with one 80-ml and two 300-ml satellite bags is described. In this procedure, a platelet concentrate was prepared from the buffy coat fraction obtained by the first centrifugation of whole blood. After centrifugation of whole blood for 5 minutes at 3500 X g, the plasma was transferred into the 300-ml bag until the interface of red cells and plasma reached a level 32 mm from the top of the bag; then approximately 70 g of plasma and buffy coat were collected into the 80-ml bag. The buffy coat fraction was centrifuged further for 5 minutes at 170 X g, and the supernatant (concentrated platelets in plasma) was transferred into the second 300-ml bag. In this blood processing, the recovery of red cells into the packed red cells and of platelets into the platelet concentrate was 93 +/- 4 percent and 52 +/- 13 percent, respectively, of the original value. White cells in the packed red cells were 70 +/- 28 X 10(7) with recovery of 32 +/- 9 percent of the original value, and the lymphocytes in the white cells were only 7 +/- 5 X 10(7) (7 +/- 4% of the original value). White cell contamination of platelet concentrate was below the threshold of white cell detection by the microcell counter (less than 300 cells per microliter of concentrate).
描述了一种从收集在带有一个80毫升和两个300毫升卫星袋的四联袋中的400毫升血液制备少白细胞红细胞的方法。在此过程中，从全血第一次离心获得的白膜层部分制备血小板浓缩物。将全血在3500×g下离心5分钟后，将血浆转移至300毫升袋中，直至红细胞与血浆的界面达到距袋顶部32毫米的水平；然后将约70克血浆和白膜层收集到80毫升袋中。将白膜层部分在170×g下进一步离心5分钟，然后将上清液（血浆中的浓缩血小板）转移至第二个300毫升袋中。在这种血液处理中，红细胞在浓缩红细胞中的回收率以及血小板在血小板浓缩物中的回收率分别为原始值的93±4%和52±13%。浓缩红细胞中的白细胞为70±28×10⁷，回收率为原始值的32±9%，白细胞中的淋巴细胞仅为7±5×10⁷（原始值的7±4%）。血小板浓缩物的白细胞污染低于微量细胞计数器检测白细胞的阈值（每微升浓缩物少于300个细胞）。
