"Petru Poni" Institute of Macromolecular Chemistry, Aleea Grigore Ghica Voda 41A, 700487, Iasi, Romania.
"Medical and Pharmaceutical Bionanotechnologies" Laboratory, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, B.P. Hasdeu 8, 050568 Bucharest, Romania.
Int J Biol Macromol. 2021 Jun 1;180:418-431. doi: 10.1016/j.ijbiomac.2021.03.082. Epub 2021 Mar 16.
Hydrogel-based wound dressings have been intensively studied as promising materials for wound healing and care. The mixed-mode thiol-acrylate photopolymerization is used in this paper for alginate/poloxamer hydrogels formation. First, the alginate was modified with thiol groups using the esterification reaction with cysteamine, and second, the terminal hydroxyl groups of poloxamer were esterified with acryloyl chloride to introduce polymerizable acrylate groups. Finally, the cross-linking reaction between the two macromers was performed to produce degradable alginate/poloxamer hydrogels. The optimum conditions for the photo-initiated reaction were studied in order to obtain high gel fractions. The resulting hydrogels have high swelling capacity in simulated physiological conditions, good elasticity and strength, and appropriate porosity, some of the physico-chemical properties required for their applications as wound dressings/patches. The biological assays show that the alginate/poloxamer hydrogels induce proliferation of human keratinocyte and have an anti-inflammatory effect on lipopolysaccharides (LPS)-activated keratinocytes by inhibiting the extracellular signal-regulated kinases (ERK)/ nuclear factor (NF)-kB/ tumor necrosis factor (TNF)-α signalling pathway. Taken together, the results showed that the chemical cross-linked alginate/poloxamer hydrogels may function as a dressing/patch applied directly on the skin lesion to heal the wound by reducing the exacerbated inflammation, the main cause of wound healing delay and local infection.
水凝胶创面敷料作为有前途的伤口愈合和护理材料受到了广泛研究。本文采用混合模式巯基-丙烯酸酯光聚合反应来制备海藻酸盐/泊洛沙姆水凝胶。首先，通过与半胱胺的酯化反应将巯基引入海藻酸盐中，然后将泊洛沙姆末端的羟基与丙烯酰氯酯化以引入可聚合的丙烯酰基团。最后，通过两个大分子之间的交联反应来制备可降解的海藻酸盐/泊洛沙姆水凝胶。为了获得高凝胶分数，研究了光引发反应的最佳条件。所得水凝胶在模拟生理条件下具有高溶胀能力、良好的弹性和强度以及适当的孔隙率，这些都是其用作创面敷料/贴剂所需的一些物理化学性质。生物测定表明，海藻酸盐/泊洛沙姆水凝胶能促进人角质形成细胞的增殖，并通过抑制细胞外信号调节激酶（ERK）/核因子（NF）-kB/肿瘤坏死因子（TNF）-α信号通路，对脂多糖（LPS）激活的角质形成细胞具有抗炎作用。综上所述，结果表明，化学交联的海藻酸盐/泊洛沙姆水凝胶可作为敷料/贴剂直接应用于皮肤损伤部位，通过减轻炎症过度反应来促进伤口愈合，而炎症过度反应是导致伤口愈合延迟和局部感染的主要原因。
