Department of Mechanical and Industrial Engineering, College of Engineering, Qatar University, 2713, Doha, Qatar; Biomedical Research Center (BRC), Qatar University, PO Box 2713, Doha, Qatar.
Department of Cardiovascular and Transplantation Surgery, Regional Central Hospital of Nancy, Lorraine University, Nancy 54500, France; Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
Biomed Pharmacother. 2021 Jun;138:111425. doi: 10.1016/j.biopha.2021.111425. Epub 2021 Mar 23.
Cardiovascular disease is one of the leading causes of mortality worldwide. Cardiac tissue engineering strategies focusing on biomaterial scaffolds incorporating cells and growth factors are emerging as highly promising for cardiac repair and regeneration. The use of stem cells within cardiac microengineered tissue constructs present an inherent ability to differentiate into cell types of the human heart. Stem cells derived from various tissues including bone marrow, dental pulp, adipose tissue and umbilical cord can be used for this purpose. Approaches ranging from stem cell injections, stem cell spheroids, cell encapsulation in a suitable hydrogel, use of prefabricated scaffold and bioprinting technology are at the forefront in the field of cardiac tissue engineering. The stem cell microenvironment plays a key role in the maintenance of stemness and/or differentiation into cardiac specific lineages. This review provides a detailed overview of the recent advances in microengineering of autologous stem cell-based tissue engineering platforms for the repair of damaged cardiac tissue. A particular emphasis is given to the roles played by the extracellular matrix (ECM) in regulating the physiological response of stem cells within cardiac tissue engineering platforms.
心血管疾病是全球范围内导致死亡的主要原因之一。专注于生物材料支架结合细胞和生长因子的心脏组织工程策略，在心脏修复和再生方面具有广阔的应用前景。在心脏微工程组织构建中使用干细胞具有分化为人心肌细胞类型的固有能力。可以为此目的使用源自各种组织的干细胞，包括骨髓、牙髓、脂肪组织和脐带。干细胞注射、干细胞球体、在合适的水凝胶中细胞包封、预制支架的使用和生物打印技术等方法处于心脏组织工程领域的前沿。干细胞微环境在维持干细胞特性和/或分化为心脏特定谱系方面起着关键作用。本综述详细概述了用于修复受损心脏组织的自体干细胞为基础的组织工程平台的微工程的最新进展。特别强调了细胞外基质 (ECM) 在调节心脏组织工程平台中干细胞的生理反应方面的作用。
