Gunjača Jerko, Carović-Stanko Klaudija, Lazarević Boris, Vidak Monika, Petek Marko, Liber Zlatko, Šatović Zlatko
Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, University of Zagreb, Zagreb, Croatia.
Centre of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv), Zagreb, Croatia.
Front Plant Sci. 2021 Mar 5;12:636484. doi: 10.3389/fpls.2021.636484. eCollection 2021.
Micronutrient malnutrition is one of the main public health problems in many parts of the world. This problem raises the attention of all valuable sources of micronutrients for the human diet, such as common bean ( L.). In this research, a panel of 174 accessions representing Croatian common bean landraces was phenotyped for seed content of eight nutrients (N, P, K, Ca, Mg, Fe, Zn, and Mn), and genotyped using 6,311 high-quality DArTseq-derived SNP markers. A genome-wide association study (GWAS) was then performed to identify new genetic sources for improving seed mineral content. Twenty-two quantitative trait nucleotides (QTN) associated with seed nitrogen content were discovered on chromosomes Pv01, Pv02, Pv03, Pv05, Pv07, Pv08, and Pv10. Five QTNs were associated with seed phosphorus content, four on chromosome Pv07, and one on Pv08. A single significant QTN was found for seed calcium content on chromosome Pv09 and for seed magnesium content on Pv08. Finally, two QTNs associated with seed zinc content were identified on Pv06 while no QTNs were found to be associated with seed potassium, iron, or manganese content. Our results demonstrate the utility of GWAS for understanding the genetic architecture of seed nutritional traits in common bean and have utility for future enrichment of seed with macro- and micronutrients through genomics-assisted breeding.
微量营养素营养不良是世界许多地区主要的公共卫生问题之一。这个问题引起了人们对人类饮食中所有重要微量营养素来源的关注，比如普通菜豆（Phaseolus vulgaris L.）。在本研究中，对代表克罗地亚普通菜豆地方品种的174份材料进行了8种养分（氮、磷、钾、钙、镁、铁、锌和锰）种子含量的表型分析，并使用6311个高质量的DArTseq衍生SNP标记进行了基因分型。随后进行了全基因组关联研究（GWAS），以确定提高种子矿物质含量的新遗传资源。在Pv01、Pv02、Pv03、Pv05、Pv07、Pv08和Pv10染色体上发现了22个与种子氮含量相关的数量性状核苷酸（QTN）。5个QTN与种子磷含量相关，4个在Pv07染色体上，1个在Pv08染色体上。在Pv09染色体上发现了1个与种子钙含量相关的显著QTN，在Pv08染色体上发现了1个与种子镁含量相关的QTN。最后，在Pv06染色体上鉴定出2个与种子锌含量相关的QTN，而未发现与种子钾、铁或锰含量相关的QTN。我们的结果证明了GWAS在理解普通菜豆种子营养性状遗传结构方面的实用性，并且对于未来通过基因组辅助育种提高种子中大量和微量营养素含量具有实用价值。
