GFZ German Research Centre for Geosciences, Section Geomicrobiology, Potsdam, Germany.
Institute for Biochemistry and Molecular Biology, Department of Chemistry, University of Hamburg, Hamburg, Germany.
Methods Mol Biol. 2021;2252:251-268. doi: 10.1007/978-1-0716-1150-0_12.
Translation is a central biological process in living cells. Ribosome profiling approach enables assessing translation on a global, cell-wide level. Extracting versatile information from the ribosome profiling data usually requires specialized expertise for handling the sequencing data that is not available to the broad community of experimentalists. Here, we provide an easy-to-use and modifiable workflow that uses a small set of commands and enables full data analysis in a standardized way, including precise positioning of the ribosome-protected fragments, for determining codon-specific translation features. The workflow is complemented with simple step-by-step explanations and is accessible to scientists with no computational background.
翻译是活细胞中的一个核心生物学过程。核糖体分析方法能够在全局范围内评估翻译。从核糖体分析数据中提取多样化的信息通常需要专门的专业知识来处理测序数据，而这对于广大实验人员来说是无法获得的。在这里，我们提供了一个易于使用和可修改的工作流程，该流程使用了一小部分命令，并以标准化的方式进行完整的数据分析，包括核糖体保护片段的精确定位，以确定密码子特异性翻译特征。该工作流程还附有简单的逐步说明，即使是没有计算背景的科学家也可以使用。
