Domingue E L, Tyndall R L, Mayberry W R, Pancorbo O C
Department of Environmental Health, Quillen-Dishner College of Medicine, East Tennessee State University, Johnson City 37614.
Appl Environ Microbiol. 1988 Mar;54(3):741-7. doi: 10.1128/aem.54.3.741-747.1988.
A study was conducted to determine the bactericidal effects of ozone and hydrogen peroxide relative to that of free chlorine on Legionella pneumophila serogroup 1. In laboratory batch-type experiments, organisms seeded at various densities were exposed to different concentrations of these biocides in demand-free buffers. Bactericidal effects were measured by determining the ability of L. pneumophila to grow on buffered charcoal-yeast extract agar supplemented with alpha-ketoglutarate. Ozone was the most potent of the three biocides, with a greater than 99% kill of L. pneumophila occurring during a 5-min exposure to 0.10 to 0.30 micrograms of O3 per ml. The bactericidal action of O3 was not markedly affected by changes in pH or temperature. Concentrations of 0.30 and 0.40 micrograms of free chlorine per ml killed 99% of the L. pneumophila after 30- and 5-min exposures, respectively. A 30-min exposure to 1,000 micrograms of H2O2 per ml was required to effect a 99% reduction of the viable L. pneumophila population. However, no viable L. pneumophila could be detected after a 24-h exposure to 100 or 300 micrograms of H2O2 per ml. Attempts were made to correlate the biocidal effects of O3 and H2O2 with the oxidation of L. pneumophila fatty acids. These tests indicated that certain biocidal concentrations of O3 and H2O2 resulted in a loss or severe reduction of L. pneumophila unsaturated fatty acids.
开展了一项研究，以确定臭氧和过氧化氢相对于游离氯对嗜肺军团菌血清1型的杀菌效果。在实验室间歇式实验中，将接种了不同密度菌的微生物暴露于无需求缓冲液中不同浓度的这些杀菌剂中。通过测定嗜肺军团菌在添加了α-酮戊二酸的缓冲活性炭酵母提取物琼脂上生长的能力来衡量杀菌效果。臭氧是三种杀菌剂中效力最强的，在每毫升暴露于0.10至0.30微克O3的5分钟内，嗜肺军团菌的杀灭率超过99%。O3的杀菌作用不受pH值或温度变化的显著影响。每毫升0.30和0.40微克的游离氯浓度分别在暴露30分钟和5分钟后杀死了99%的嗜肺军团菌。每毫升1000微克的H2O2暴露30分钟才能使嗜肺军团菌活菌数量减少99%。然而，每毫升暴露于100或300微克H2O2 24小时后，未检测到存活的嗜肺军团菌。研究人员试图将O3和H2O2的杀菌效果与嗜肺军团菌脂肪酸的氧化联系起来。这些测试表明，O3和H2O2的某些杀菌浓度会导致嗜肺军团菌不饱和脂肪酸的损失或严重减少。
