分子级别的轴向定位通过重复的光学选择性曝光实现。
Molecular-scale axial localization by repetitive optical selective exposure.
机构信息
National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Physical Science Laboratory, Huairou National Comprehensive Science Center, Beijing, China.
出版信息
Nat Methods. 2021 Apr;18(4):369-373. doi: 10.1038/s41592-021-01099-2. Epub 2021 Apr 1.
We introduce an axial localization with repetitive optical selective exposure (ROSE-Z) method for super-resolution imaging. By using an asymmetric optical scheme to generate interference fringes, a <2 nm axial localization precision was achieved with only ~3,000 photons, which is an approximately sixfold improvement compared to previous astigmatism methods. Nanoscale three-dimensional and two-color imaging was demonstrated, illustrating how this method achieves superior performance and facilitates the investigation of cellular nanostructures.
我们提出了一种轴向定位的重复光学选择曝光（ROSE-Z）方法，用于超分辨率成像。通过使用非对称光学方案产生干涉条纹，我们实现了<2nm 的轴向定位精度，仅需约 3000 个光子，与之前的像散方法相比提高了约六倍。我们展示了纳米级三维和双色成像，说明了这种方法如何实现卓越的性能并促进对细胞纳米结构的研究。
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