Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, 38163, United States.
Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, Memphis, TN, 38163, United States; Department of Endodontics, Chi Mei Medical Center, Liouying, Tainan, Taiwan; Department of Endodontics, Chi Mei Medical Center, Yongkang, Tainan, Taiwan.
J Dent. 2021 Jun;109:103655. doi: 10.1016/j.jdent.2021.103655. Epub 2021 Mar 30.
INTRODUCTION/OBJECTIVE: The tailored amorphous multi-porous (TAMP) material fabrication technology has led to a new class of bioactive materials possessing versatile characteristics. It has not been tested for dental applications. Thus, we aimed to assess its biocompatibility and ability to regenerate dental mineral tissue.
30CaO-70SiO model TAMP discs were fabricated by a sol-gel method followed by in vitro biocompatibility testing with isolated human or mini-swine dental pulp stem cells (DPSCs). TAMP scaffolds were tested in vivo as a pulp exposure (pin-point, 1 mm, 2 mm, and entire pulp chamber roof) capping material in the molar teeth of mini-swine.
The in vitro assays showed that DPSCs attached well onto the TAMP discs with comparable viability to those attached to culture plates. Pulp capping tests on mini-swine showed that after 4.5 months TAMP material was still present at the capping site, and mineral tissue (dentin bridge) had formed in all sizes of pulp exposure underneath the TAMP material.
TAMP calcium silicate is biocompatible with both human and swine DPSCs in vitro and with pulp in vivo, it may help regenerate the dentin bridge after pulp exposure.
简介/目的：定制的无定形多孔隙（TAMP）材料制造技术产生了一类具有多种特性的新型生物活性材料。尚未对其在牙科中的应用进行测试。因此，我们旨在评估其生物相容性和再生牙矿化组织的能力。
采用溶胶-凝胶法制备 30CaO-70SiO 模型 TAMP 圆盘，随后用分离的人或小型猪牙髓干细胞（DPSCs）进行体外相容性测试。TAMP 支架作为牙髓暴露（针状，1mm、2mm 和整个牙髓腔顶）的覆盖材料，在小型猪的磨牙中进行体内测试。
体外检测表明，DPSCs 很好地附着在 TAMP 盘上，与附着在培养板上的细胞活力相当。在小型猪上进行的牙髓覆盖试验表明，4.5 个月后，TAMP 材料仍存在于覆盖部位，在 TAMP 材料下方所有大小的牙髓暴露处都形成了矿化组织（牙本质桥）。
TAMP 硅酸钙在体外与人和猪的 DPSCs 具有生物相容性，在体内与牙髓具有生物相容性，它可能有助于在牙髓暴露后再生牙本质桥。
