Alpsoy Aktan, Sood Surbhi, Dykhuizen Emily C
Purdue University Interdisciplinary Life Science Graduate Program (PULSe), Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.
Purdue University Center for Cancer Research, Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47907, USA.
Biology (Basel). 2021 Mar 27;10(4):272. doi: 10.3390/biology10040272.
In higher order organisms, the genome is assembled into a protein-dense structure called chromatin. Chromatin is spatially organized in the nucleus through hierarchical folding, which is tightly regulated both in cycling cells and quiescent cells. Assembly and folding are not one-time events in a cell's lifetime; rather, they are subject to dynamic shifts to allow changes in transcription, DNA replication, or DNA damage repair. Chromatin is regulated at many levels, and recent tools have permitted the elucidation of specific factors involved in the maintenance and regulation of the three-dimensional (3D) genome organization. In this review/perspective, we aim to cover the potential, but relatively unelucidated, crosstalk between 3D genome architecture and the ATP-dependent chromatin remodelers with a specific focus on how the architectural proteins CTCF and cohesin are regulated by chromatin remodeling.
在高等生物中，基因组组装成一种名为染色质的蛋白质密集结构。染色质通过分级折叠在细胞核中进行空间组织，这种折叠在循环细胞和静止细胞中都受到严格调控。组装和折叠并非细胞生命周期中的一次性事件；相反，它们会发生动态变化，以允许转录、DNA复制或DNA损伤修复发生改变。染色质在多个层面受到调控，最近的技术工具使我们能够阐明参与三维（3D）基因组组织维持和调控的特定因子。在这篇综述/观点文章中，我们旨在探讨3D基因组结构与ATP依赖的染色质重塑因子之间潜在但相对未被阐明的相互作用，特别关注结构蛋白CTCF和黏连蛋白如何受到染色质重塑的调控。
