Institut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, IBS, 71 Avenue des Martyrs, F-38044 Grenoble, France.
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK.
Biomolecules. 2021 Mar 25;11(4):495. doi: 10.3390/biom11040495.
Chemotactic responses in motile bacteria are the result of sophisticated signal transduction by large, highly organized arrays of sensory proteins. Despite tremendous progress in the understanding of chemosensory array structure and function, a structural basis for the heightened sensitivity of networked chemoreceptors is not yet complete. Here, we present cryo-electron tomography visualisations of native-state chemosensory arrays in minicells. Strikingly, these arrays appear to exhibit a p2-symmetric array architecture that differs markedly from the p6-symmetric architecture previously described in . Based on this data, we propose molecular models of this alternative architecture and the canonical p6-symmetric assembly. We evaluate our observations and each model in the context of previously published data, assessing the functional implications of an alternative architecture and effects for future studies.
运动细菌的趋化反应是由大量高度组织化的感觉蛋白通过复杂的信号转导产生的。尽管在理解化学感应阵列的结构和功能方面取得了巨大进展，但网络化化学感受器的高灵敏度的结构基础尚未完全清楚。在这里，我们展示了原生状态化学感应阵列在 minicells 中的低温电子断层扫描可视化结果。引人注目的是，这些阵列似乎表现出 p2 对称的阵列结构，与以前在 中描述的 p6 对称结构明显不同。基于这些数据，我们提出了这种替代结构和典型的 p6 对称组装的分子模型。我们根据以前发表的数据评估我们的观察结果和每个模型，评估替代结构的功能意义和对未来研究的影响。
