School of Pharmaceutical Sciences, Jilin University, Fujin Road 1266, Changchun 130021, China.
Molecules. 2021 Mar 25;26(7):1855. doi: 10.3390/molecules26071855.
Six parts of lotus (seeds, leaves, plumule, stamens, receptacles and rhizome nodes) are herbal medicines that are listed in the Chinese Pharmacopoeia. Their indications and functions have been confirmed by a long history of clinical practice. To fully understand the material basis of clinical applications, UPLC-QToF-MS combined with the UNIFI platform and multivariate statistical analysis was used in this study. As a result, a total of 171 compounds were detected and characterized from the six parts, and 23 robust biomarkers were discovered. The method can be used as a standard protocol for the direct identification and prediction of the six parts of lotus. Meanwhile, these discoveries are valuable for improving the quality control method of herbal medicines. Most importantly, this was the first time that alkaloids were detected in the stamen, and terpenoids were detected in the cored seed. The stamen is a noteworthy part because it contains the greatest diversity of flavonoids and terpenoids, but research on the stamen is rather limited.
六部分荷花（莲子、荷叶、胚芽、雄蕊、花托和根茎节点）都是中草药，被收录在中国药典中。它们的适应证和功能已经被长期的临床实践所证实。为了充分了解临床应用的物质基础，本研究采用 UPLC-QToF-MS 结合 UNIFI 平台和多变量统计分析方法。结果，从六部分共检测到并鉴定了 171 种化合物，并发现了 23 个稳健的生物标志物。该方法可作为直接鉴定和预测荷花六部分的标准方案。同时，这些发现对于改进中草药的质量控制方法具有重要价值。最重要的是，这是首次在雄蕊中检测到生物碱，在去芯种子中检测到萜类化合物。雄蕊是一个值得关注的部分，因为它含有最多样化的黄酮类化合物和萜类化合物，但对雄蕊的研究却相当有限。
