枝动霉素A和B，从一株sp. 195334CR中分离得到的环六肽。

Amycolatomycins A and B, Cyclic Hexapeptides Isolated from an sp. 195334CR.

作者信息

Primahana Gian, Risdian Chandra, Mozef Tjandrawati, Wink Joachim, Surup Frank, Stadler Marc

机构信息

Department Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), Inhoffenstrasse 7, 38124 Braunschweig, Germany.

Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), Kawasan Puspiptek, Serpong, Tangerang Selatan 15314, Indonesia.

出版信息

Antibiotics (Basel). 2021 Mar 5;10(3):261. doi: 10.3390/antibiotics10030261.

DOI:10.3390/antibiotics10030261

PMID:33807584

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8002008/

Abstract

The rare actinobacterium sp. strain 195334CR was found to produce previously undescribed cyclic hexapeptides, which we named amycolatomycin A and B ( and ). Their planar structures were determined by high-resolution mass spectrometry as well as extensive 1D and 2D NMR spectroscopy, while the absolute stereochemistry of its amino acids were determined by Marfey's method. Moreover, and differ by the incorporation of l-Ile and l--Ile, respectively, whose FDVA (Nα-(2,4-Dinitro-5-fluorphenyl)-L-valinamide) derivatives were separated on a C column. Their hallmark in common is a unique 2,6-dichloro-tryptophan amino acid unit. Amycolatomycin A () exhibited weak activity against DSM 10 (minimum inhibitory concentration (MIC) = 33.4 µg/mL).

摘要

人们发现稀有放线菌菌株195334CR能产生此前未被描述的环六肽，我们将其命名为枝动霉素A和B（和）。它们的平面结构通过高分辨率质谱以及广泛的一维和二维核磁共振光谱确定，而其氨基酸的绝对立体化学通过马尔费方法确定。此外，和的区别分别在于掺入了L-异亮氨酸和L- -异亮氨酸，其FDVA（Nα-（2,4-二硝基-5-氟苯基）-L-缬氨酰胺）衍生物在C柱上分离。它们共有的标志是一个独特的2,6-二氯色氨酸氨基酸单元。枝动霉素A（）对 DSM 10表现出较弱的活性（最低抑菌浓度（MIC） = 33.4 µg/mL）。