Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea.
CHA Bundang Medical Center, Department of Obstetrics and Gynecology, School of Medicine, CHA University, Seongnam 13496, Korea.
Int J Mol Sci. 2021 Mar 16;22(6):3021. doi: 10.3390/ijms22063021.
Recurrent implantation failure (RIF) refers to the occurrence of more than two failed in vitro fertilization-embryo transfers (IVF-ETs) in the same individual. RIF can occur for many reasons, including embryo characteristics, immunological factors, and coagulation factors. Genetics can also contribute to RIF, with some single-nucleotide variants (SNVs) reported to be associated with RIF occurrence. We examined SNVs in a long non-coding RNA, homeobox () transcript antisense RNA (), which is known to affect cancer development. regulates epigenetic outcomes through histone modifications and chromatin remodeling. We recruited 155 female RIF patients and 330 healthy controls, and genotyped SNVs, including rs4759314, rs920778, rs7958904, and rs1899663, in all participants. Differences in these SNVs were compared between the patient and control groups. We identified significant differences in the occurrence of heterozygous genotypes and the dominant expression model for the rs1899663 and rs7958904 SNVs between RIF patients and control subjects. These variants were associated with serum hemoglobin (Hgb), luteinizing hormone (LH), total cholesterol (T. chol), and blood urea nitrogen (BUN) levels, as assessed by analysis of variance (ANOVA). We analyzed the four SNVs and found significant differences in haplotype patterns between RIF patients and healthy controls. The results of this study showed that is not only associated with the development of cancer but also with pregnancy-associated diseases. This study represents the first report showing that is correlated with RIF.
反复着床失败（RIF）是指同一个体发生两次或两次以上的体外受精-胚胎移植（IVF-ET）失败。RIF 的发生有很多原因，包括胚胎特征、免疫因素和凝血因素。遗传学也可能导致 RIF，一些单核苷酸变异（SNV）已被报道与 RIF 的发生有关。我们研究了长非编码 RNA 同源盒（）转录反义 RNA（）中的 SNV，该 RNA 已知会影响癌症的发展。通过组蛋白修饰和染色质重塑来调节表观遗传结果。我们招募了 155 名女性 RIF 患者和 330 名健康对照者，并对所有参与者进行了 rs4759314、rs920778、rs7958904 和 rs1899663 等 SNV 的基因分型。比较了这些 SNV 在患者组和对照组之间的差异。我们发现 rs1899663 和 rs7958904 SNV 的杂合基因型和显性表达模型在 RIF 患者和对照组之间存在显著差异。这些变体与血清血红蛋白（Hgb）、促黄体生成素（LH）、总胆固醇（T. chol）和血尿素氮（BUN）水平有关，通过方差分析（ANOVA）进行评估。我们分析了这四个 SNV，发现 RIF 患者和健康对照组之间的单倍型模式存在显著差异。这项研究的结果表明，不仅与癌症的发展有关，还与妊娠相关疾病有关。本研究首次表明与 RIF 相关。
