Department of Infectious Diseases, Children's Hospital of Fudan University, Shanghai, China.
Department of Microbiology, Changning District Center for Disease Control and Prevention, Shanghai, China.
PLoS One. 2021 Apr 8;16(4):e0249888. doi: 10.1371/journal.pone.0249888. eCollection 2021.
Diarrhoea remains a major cause of childhood morbidity and mortality worldwide. This study aimed to monitor the aetiology of acute diarrhoea in children in Shanghai. Paediatric outpatients with acute diarrhoea were enrolled in the study from Jan 2015 to Dec 2018. Faecal samples were collected for testing. Enteric bacteria were identified and typed by culture and serotyping, respectively. Enteric viruses were identified by real-time PCR. Enteric pathogens were identified in 1572 (58.4%) of the 2692 enrolled children with acute diarrhoea. Viruses were detected more frequently than bacteria (41.3% versus 25.0%). Nontyphoidal Salmonella spp. (NTS) was the most common (10.3%) bacteria isolated, followed by enteropathogenic Escherichia coli (EPEC) (6.5%), enteroaggregative Escherichia coli (EAEC) (6.2%), Campylobacter spp. (3.6%), enterotoxigenic Escherichia coli (ETEC) (1.1%), Shigella spp. (0.2%), and enterohemorrhagic Escherichia coli (EHEC) (0.1%). Rotavirus was the most common (16.0%) virus detected, followed by norovirus (15.5%), adenovirus (7.2%), sapovirus (3.0%) and astrovirus (2.7%). Rotavirus, norovirus and NTS were the major pathogens responsible for diarrhoea in Shanghainese children. Improving uptake of the rotavirus vaccine and strengthening foodborne-pathogen prevention will aid in reducing the burden of diarrhoeal disease in children in Shanghai.
腹泻仍是全世界儿童发病和死亡的主要原因。本研究旨在监测上海地区儿童急性腹泻的病因。2015 年 1 月至 2018 年 12 月，我们招募了患有急性腹泻的儿科门诊患儿。采集粪便样本进行检测。通过培养和血清分型分别鉴定肠道细菌并分型，通过实时 PCR 鉴定肠道病毒。在 2692 例急性腹泻患儿中，有 1572 例（58.4%）患儿肠道病原体检测阳性。病毒的检出率高于细菌（41.3%比 25.0%）。非伤寒沙门氏菌（NTS）是最常见的分离菌（10.3%），其次是肠致病性大肠杆菌（EPEC）（6.5%）、肠聚集性大肠杆菌（EAEC）（6.2%）、弯曲菌属（3.6%）、肠产毒性大肠杆菌（ETEC）（1.1%）、志贺菌属（0.2%）和肠出血性大肠杆菌（EHEC）（0.1%）。轮状病毒是最常见的病毒（16.0%），其次是诺如病毒（15.5%）、腺病毒（7.2%）、星状病毒（3.0%）和星状病毒（2.7%）。轮状病毒、诺如病毒和 NTS 是导致上海儿童腹泻的主要病原体。提高轮状病毒疫苗的接种率和加强食源性病原体的预防将有助于降低上海儿童腹泻病的负担。
